We narrowed to 11,590 results for: 110

1. TAL2268

   Plasmid

   #36705

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Left (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

2. TAL2269

   Plasmid

   #36706

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FOXO3-TALEN_Right (Foxo3 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pMOD_A0802

   Plasmid

   #91023

   Purpose

   Module A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_dead (D10A + H840A)
   

   Use

   CRISPR

   Mutation

   D10A, H840A

   Promoter

   AtUbi10

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Non-targeting CRISPRi plasmid

   Plasmid

   #154891

   Purpose

   Human non-targeting CRISPRi gRNA

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

   Insert

   Nontargeting gRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAtCas9-dead_1

   Plasmid

   #91165

   Purpose

   Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   AtCas9_D10A+H840A (dead)
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJG493

   Plasmid

   #91197

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gUbi1+gUbi8+donor
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. 815 pSG5L HA PTEN delta274-342

   Plasmid

   #10738

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 274-342.

   Available Since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pDEST myc MOV10 D645N

   Plasmid

   #51720

   Purpose

   plasmid expresses myc-tagged MOV10 D645N

   Depositor

   Markus Landthaler

   Article

   Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

   Insert

   MOV10 D645N (MOV10 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   D645N

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTaCas9_5

   Plasmid

   #91168

   Purpose

   Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   TaCas9
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCas9-H840A_2

    Plasmid

    #91163

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A (nickase)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    H840A

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GAL 25Q+ProCFPp303

    Plasmid

    #15574

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt25Q + Pro (HTT Human)
    

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pMDC84-SEIPIN3-Ndelta

    Plasmid

    #96981

    Purpose

    Express N-terminal truncated Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Tags

    6xHis and GFP

    Expression

    Plant

    Mutation

    A segment of 507 nucleotides are deleted from the…

    Promoter

    35S

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221-RAP1

    Plasmid

    #84525

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Mutation

    no stop codon

    Promoter

    none

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSBL_DK198

    Plasmid

    #156425

    Purpose

    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

    Depositor

    Stephen Brohawn

    Article

    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

    Insert

    SARS-CoV-2 3a protein (aa 42-275)
    

    Tags

    sfGFP-His

    Expression

    Insect

    Mutation

    aa 42-275

    Promoter

    Polyhedrin

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJG486

    Plasmid

    #91195

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi1+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. GAL HA25QDProCFPp303

    Plasmid

    #15578

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. GAL HA103QDProCFPp303

    Plasmid

    #15579

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Tags

    CFP and HA

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pWZL N-Myc-hPOT1_IRES_hygro

    Plasmid

    #166406

    Purpose

    express hPOT1 in mammalian cells

    Depositor

    Agnel Sfeir

    Article

    Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

    Insert

    hPOT1 (POT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUB6_ATR

    Plasmid

    #84016

    Purpose

    ATR minigene to test splicing event

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    ATR (ATR Human)
    

    Expression

    Mammalian

    Promoter

    UB6

    Available Since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid

    #46839

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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