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pBR25 CFAP36-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238226

Purpose

CRISPR donor plasmid for C-terminal tagging of CFAP36 and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Publication

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab965-PX459-BRCA1-Nterm-guide1

Plasmid

#249005

Purpose

This plasmid expresses the Cas9/gRNA that will target the HBEGF gene locus

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

Cas9

Use

CRISPR

Tags

3xFLAG

Promoter

chicken beta-actin promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/Integrin-b5-FL-SGx3-Halo9

Plasmid

#249166

Purpose

Mammalian expression vector of human ITGB5 fused with Halo9-tag

Depositor

Pakorn Kanchanawong

Publication

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Integrin beta-5 (ITGB5 Human)

Tags

Halo9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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mIFP-Talin1(1–2493)

Plasmid

#249169

Purpose

Mammalian expression vector of truncated talin1 lacks C-terminal dimerization domain (ΔDD)

Depositor

Pakorn Kanchanawong

Publication

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Talin1 (TLN1 Human)

Tags

mIFP

Expression

Mammalian

Mutation

Deleted amino acids 2494–2541

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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mApple-Talin1(1–1973)-pdDronpa1.2

Plasmid

#249167

Purpose

Mammalian expression vector of talin1 N-terminal fragment fused with pdDronpa1.2

Depositor

Pakorn Kanchanawong

Publication

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Talin1 (TLN1 Human)

Tags

mApple and pdDronpa1.2

Expression

Mammalian

Mutation

Deleted amino acids 1974–2541

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pdDronpa1.2-Talin1(1974–2541)-mIFP

Plasmid

#249168

Purpose

Mammalian expression vector of talin1 C-terminal fragment fused with pdDronpa1.2

Depositor

Pakorn Kanchanawong

Publication

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

Talin1 (TLN1 Human)

Tags

mIFP and pdDronpa1.2

Expression

Mammalian

Mutation

Deleted amino acids 1–1973

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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DD-N-nLuc-QtSig

Plasmid

#236777

Purpose

Expresses the luminescent protein nLuc fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Publication

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N-nLuc-QtSig

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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HA_DD-N-mTagBFP2-QtSig

Plasmid

#236776

Purpose

Expresses the blue fluorescent protein mTagBFP2 fused to the encapsulation signal for QtEnc and fused to a degron signal for proteasomal degradation when not encapsulated.

Depositor

Gil Westmeyer

Publication

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

DD-N, TagBFP, QtSig

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pColE1-MP-Violacein

Plasmid

#240205

Purpose

Violacein biosynthesis pathway induced by CRISPRa

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

vioA-vioB-vioC-vioD-vioE

Expression

Bacterial

Promoter

J3-DA9-UP1-MP1

Available since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pSC-MRscRNA

Plasmid

#240211

Purpose

Yeast vector expressing theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TetO

Expression

Yeast

Promoter

SNR52

Available since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MCP-VPR

Plasmid

#240213

Purpose

Mammalian vector for transient expression of MCP-VPR

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

MCP-VPR

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-SNAPf-Sec61b

Plasmid

#247295

Purpose

Mammalian expression of the SNAPf-tagged ER reporter SEC61b; to label ER membranes.

Depositor

Jennifer Lippincott-Schwartz

Publication

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

SNAPf-Sec61b (SEC61B Human)

Tags

SnapTag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOP576

Plasmid

#85943

Purpose

Temperature sensitive plasmid based on pDK46. Expresses dCas9 from proD. Serves as a control for pOP587 - pOP594

Depositor

Timothy Lu

Publication

Purcell et al Nat Commun. 2018 Nov 22;9(1):4942. doi:

Insert

dCas9

Expression

Bacterial

Available since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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P-d22bp

Plasmid

#235657

Purpose

B. thetaiotaomicron fusA2 expression reporter lacking 22bp Cur binding site

Depositor

Guy Townsend

Publication

Kabonick et al bioRxiv [Preprint]. 2024 Nov 13:2024.11.

Insert

Bacteroides thetaiotaomicron fusA2 promoter lacking the 22bp Cur binding site

Mutation

The B. thetaiotaomicron fusA2 promoter lacking &q…

Available since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_FL Gcn4

Plasmid

#232955

Purpose

Galactose iduced expression of full-length Gcn4 in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 SATtoG KtoRHA

Plasmid

#232994

Purpose

Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 WT 44merHA

Plasmid

#232988

Purpose

Galactose iduced expression of Gcn4 WT 44merHA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WT 44mer

Tags

3xHA and TEV cleavage site

Expression

Yeast

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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