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pEMS1496

Plasmid

#29244

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple25 (CCKBR Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Oct. 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pBS Alpha 2 globin

Plasmid

#19231

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Alpha 2 globin (HBA1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pAG145 HIPK1 3'UTR

Plasmid

#12003

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pAG184 NEK6 3'UTR

Plasmid

#12014

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N17 3'UTR (NEK6 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREB3L2

Plasmid

#207207

Purpose

doxycycline-inducible expression of Human CREB3L2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-CREB3L2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZEB2

Plasmid

#207203

Purpose

doxycycline-inducible expression of Human ZEB in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ZEB2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-NFATC2

Plasmid

#207191

Purpose

doxycycline-inducible expression of Human NFATC2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-NFATC2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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NLuc-His10

Plasmid

#234567

Purpose

Bacterially expressed NLuc for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

Nano luciferase

Tags

T7 gene 10 leader peptide and Thrombin site-10xHi…

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

T7

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-YAP-MAML2

Plasmid

#237674

Purpose

For overexpression of mEGFP-YAP-MAML2

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

YAP-MAML2 (YAP1, MAML2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shVgf-CWB

Plasmid

#223658

Purpose

Expresses hM3D(Gq) and a shRNA targetting VGF in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shVGF

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pStreptagII 3C SMO

Plasmid

#234995

Purpose

Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

SMO (SMO Human)

Tags

Streptag-II, HRV 3C site

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_circHUWE1_1

Plasmid

#215236

Purpose

Supression of shcircHUWE1(19,20)_1 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circHUWE1 shRNA 1 (HUWE1 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF8

Plasmid

#232668

Purpose

transient expresses IRF8 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chen et al HGG Adv. 2026 May 25:100630. doi: 10.101

Insert

IRF8 (IRF8 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-pDEST_TMEM192_BirA_Flag

Plasmid

#227958

Purpose

BioID experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

TMEM192 (TMEM192 Human)

Tags

BirA-FLAG

Expression

Mammalian

Mutation

R118G in BirA

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW00011

Plasmid

#222439

Purpose

Level 0, CDSns module (AATG-TTCG) containing S. cerevisiae CUP2.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CUP2 (Saccharomyces cerevisiae) (CUP2 Budding Yeast)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag

Plasmid

#224894

Purpose

Mammalian expression of LgBiT-MYPT1(1--314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

LgBiT-MYPT1(1-314)KAKA-Flag

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEZYeGFP-SARS-CoV-2E

Plasmid

#224580

Purpose

To express a GFP tagged version of the Envelope protein of SARS-CoV-2 in mammalian cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Int J Mol Sci. 2023 Aug 14;24(16):12793.

Insert

SARS-CoV-2E (E SARS-CoV-2)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-E46K

Plasmid

#181723

Purpose

Galactose inducible expression of Alpha-synuclein-E46K

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

E46K

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-Alpha-synuclein-H50Q

Plasmid

#181724

Purpose

Galactose inducible expression of Alpha-synuclein-H50Q

Depositor

Alejandro Chavez

Insert

Alpha-synuclein (SNCA Human)

Expression

Yeast

Mutation

H50Q

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pIBA2-Int-SpyTag

Plasmid

#198038

Purpose

Expression plasmid for producing SpyTagged stalled secretion variant of intimin

Depositor

Jack Leo

Article

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

lacks native signal peptide (amino acids 1-39)

Promoter

tet

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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