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We narrowed to 18,247 results for: multi

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Showing: 9081 - 9100 of 18247 results

  3. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Emmanuele Severi, Gavin Thomas
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  6. pZyxin-mMaple2

    Plasmid
    #101150
    Purpose
    Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate
    Depositor
    Xiaowei Zhuang
    Publication
    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized mMaple2 fused to zyxin
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    101150-plasmid-map-sequence-id-205974

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  7. pYZ172

    Plasmid
    #98409
    Purpose
    Donor DNA plasmid to introduce lys9 deletion in S. pombe. Linearization with Not1 digestion.
    Depositor
    Jef Boeke
    Publication
    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.
    Inserts
    upstream homologous region to delete lys9 in genome by recombination
    downstream homologous region to delete lys9 in genome by recombination
    Use
    CRISPR and Synthetic Biology
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    98409-plasmid-map-sequence-id-198900

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  9. [GFP][IRES][Bla]

    Plasmid
    #97194
    Purpose
    Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.
    Depositor
    Edward Boyden
    Publication
    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta-Lactamase
    GFP
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    97194-plasmid-map-sequence-id-197878

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  10. pY036_ATP1A1_G5

    Plasmid
    #86618
    Purpose
    Expresses the ATP1A1 G5 crRNA in combination with AsCpf1-3xHA to target ATP1A1 intron 4. U6-crRNA(ATP1A1 G5)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86618-plasmid-map-sequence-id-168853

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  11. pFastBacDual with LFn + PrgJ

    Plasmid
    #84869
    Purpose
    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Russell Vance
    Publication
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    PrgJ
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    84869-plasmid-map-sequence-id-162908

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  14. pTSlb-R756K

    Plasmid
    #60731
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S
    Depositor
    Matthew Bennett
    Publication
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Residues 1-180 of split T7 RNAP and Residues 180-…
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    60731-plasmid-map-sequence-id-99358

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Showing: 9081 - 9100 of 18247 results