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pcDNA3.2/V5 mmu-mir-1941

Plasmid

#26341

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1941 (Mir1941 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1947

Plasmid

#26342

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1947 (Mir1947 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1968

Plasmid

#26344

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1968 (Mir1968 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/V5 mmu-mir-1839

Plasmid

#26345

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-1839 (Mir1839 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pENTR4_EWSR1_C2G_R650G

Plasmid

#26371

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Entry vector

Mutation

R650G

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET-17b_K490clm_E215C

Plasmid

#169995

Purpose

Kinesin KIF5B K490 cysteine light mutant with E215C mutation for single cysteine labeling

Depositor

Ron Vale

Article

Niekamp et al Proc Natl Acad Sci U S A. 2019 Mar 5;116

Insert

kinesin family member 5B, isoform CRA_a

Tags

6xHIS

Expression

Bacterial

Mutation

C7S, C65A, C168A, C174S, E215C, C294A, C330S, C42…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pPGK-SB13

Plasmid

#236078

Purpose

SB13 transposase, for use with Sleeping Beauty transponson plasmids

Depositor

Masashi Narita

Article

Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

Insert

SB13 transposase

Use

Sleeping beauty (sb) transposase plasmid

Promoter

PGK

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-His-CD276

Plasmid

#232662

Purpose

transient expresses CD276 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

CD276 (CD276 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pH3mCHSH2

Plasmid

#101053

Purpose

This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.

Depositor

Jennifer Lodge

Article

Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108

Insert

Cryptococcus mCherry expression plasmid

Expression

Yeast

Promoter

Cryptococcus Histone3 promoter

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Tags

Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSC5GGv1

Plasmid

#194645

Purpose

pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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lenti dCAS-VP64_Blast

Plasmid

#61425

Purpose

3rd generation lenti vector encoding dCAS9-VP64 with 2A Blast resistance marker (EF1a-NLS-dCas9(N863)-VP64-2A-Blast-WPRE)

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Lentiviral Prep

Insert

dCAS9(D10A, N863A)-VP64_2A_Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-KO

Plasmid

#17603

Purpose

Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

KO: all lysines mutated to arginines

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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EGFP-OSBP-PH

Plasmid

#49571

Purpose

expresses the plekstrin homology domain of OSBP in mammalian cells

Depositor

Marci Scidmore

Article

Moorhead et al Infect Immun. 2010 May;78(5):1990-2007.

Insert

OSBP-PH (AA 91-179) (OSBP Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE NFkB-TA-LUC-UBC-GFP-W

Plasmid

#49343

Purpose

pHAGE NFkB-TA-LUC-UBC-GFP-W

Depositor

Darrell Kotton

Article

Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

Inserts

eGFP (eGFP )
Luciferase
NFkB consensus binding sequence
TA
UBC

Use

Lentiviral

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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138-dCas9-Dnmt3a

Plasmid

#84570

Purpose

PiggyBac transposon system construct with dCas9-Dnmt3a

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Expression

Mammalian

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTYMS

Plasmid

#217430

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-mpeg1.1

Plasmid

#75023

Purpose

Can be used to drive expression in zebrafish microglia.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

macrophage expressed 1, tandem duplicate 1 (mpeg1.1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

mpeg1.1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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TurboID-MCS MSCV-IRES-GFP

Plasmid

#207958

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the N-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_FUS

Plasmid

#26374

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only