We narrowed to 63,742 results for: cloning
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Plasmid#70513PurposeGateway ORF clone of human RASA2 [NM_006506.3] with stop codon (for native or N-terminal fusions)DepositorInsertRASA2 (RASA2 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E171 Hs.PIK3R1
Plasmid#70455PurposeGateway ORF clone of human PIK3R1 [NM_181523.2] with stop codon (for native or N-terminal fusions)DepositorInsertPIK3R1 (PIK3R1 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E084 Hs.FGFR1-nostop
Plasmid#70368PurposeGateway ORF clone of human FGFR1 [NM_001174063.1] without stop codon (for C-terminal fusions)DepositorInsertFGFR1 (FGFR1 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E085 Hs.FGFR2
Plasmid#70369PurposeGateway ORF clone of human FGFR2 [NM_000141.4] with stop codon (for native or N-terminal fusions)DepositorInsertFGFR2 (FGFR2 Human)
UseGateway CloningAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-gRNA2.1
Plasmid#170512PurposegRNA cloning vector containing a U6:3 promoter and a gRNA2.1 scaffold.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2T-CAG-MCS-P2A-GFP-PuroR
Plasmid#107186PurposeBase plasmid for cloning gene duplication alleles with eGFP reporter.DepositorTypeEmpty backboneAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorPublicationInsertT7+RBS+His6+thrombin
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorPublicationTypeEmpty backboneExpressionBacterialAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA
Plasmid#109314PurposePlasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rat brain inositol 1,4,5-trisphosphate 3-kinase A
Plasmid#122155Purposerat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)DepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
ExpressionBacterialAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus
Plasmid#214678PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLM433
Plasmid#217293PurposeFor creating CyanoTag vectors by Golden Gate cloning (BspQI version)DepositorTypeEmpty backboneUseSynthetic BiologyTagsmNeonGreen - 3x FLAGAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B52 + FANCM sgSTOP
Plasmid#100710PurposeB52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
B52 + CHEK2 sgSTOP
Plasmid#100712PurposeB52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVP096
Plasmid#222043PurposepGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.DepositorInsertmRFP1e
UseSynthetic Biology; Greengate compatible cloning v…Available sinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pM.010
Plasmid#228696PurposeC terminal Strep affinity tag with TEV cleavage siteDepositorInsertTEV-Strep
ExpressionInsectMutationWTAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.009
Plasmid#228695PurposeN terminal Strep affinity tag with TEV cleavage siteDepositorInsertStrep-TEV
ExpressionInsectMutationWTAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB.BIG1e
Plasmid#228679PurposeMoclo Baculo toolkit level 2 backbone (biGBac compatible)DepositorTypeEmpty backboneExpressionInsectMutationGentR R98KAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMB.BIG1d
Plasmid#228678PurposeMoclo Baculo toolkit level 2 backbone (biGBac compatible)DepositorTypeEmpty backboneExpressionInsectMutationGentR R98KAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only