We narrowed to 17,816 results for: Met;

1. pAAV-P3-Alb-mNG

   Plasmid

   #183460

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-CIB1-mCerulean-MP

   Plasmid

   #58366

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

   Plasmid

   #47631

   Purpose

   An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   eNpHR3.0
   

   Use

   AAV and Cre/Lox

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129027

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCardinal-Farnesyl-5

   Plasmid

   #56159

   Purpose

   Localization: Plasma Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Farnesyl (HRAS Human)
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   encodes final 20 aa of NM_001130442.1

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pTetON-DHFRdd-SpvB; CMV-mCherry

   Plasmid

   #89463

   Purpose

   Tet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Use

   Tags

   E. coli DHFRdd destabilization domain and mCherry…

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   TRE3G

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

   Plasmid

   #47904

   Purpose

   An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   eNpHR3.0
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

   Plasmid

   #55645

   Purpose

   Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV5, AAV8, and AAV9

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. LMP AMPK alpha1 hp1

   Plasmid

   #89489

   Purpose

   shRNA against AMPK alpha1 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Mouse, Human)
   

   Use

   Mouse Targeting and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (The GFP-Puro is from a PGK promoter; the shRN…

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pETM6-G6-vioABE-4A6-vioC

    Plasmid

    #73439

    Purpose

    Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    PG6-vioABE-P4A6-vioC
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PG6 and P4A6 (orthogonal T7-lac variants)

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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