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pKM499

Plasmid

#134216

Purpose

Baculovirus expression of CENP-U CENP-Q CENP-OP

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPU + CENPQ+ CENPOP

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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PX458-gR2A_ISO

Plasmid

#124808

Purpose

Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a> plasmids to convert expression of rat IgG2a hybridomas to isotype of choice.

Depositor

Ferenc Scheeren, Martijn Verdoes

Article

van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

Insert

hSPCas9

Tags

3XFLAG and GFP

Expression

Mammalian

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-mRFP

Plasmid

#128126

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen (benzoate responsive promoter)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-mWasabi-nat

Plasmid

#120331

Purpose

Bacterial mWasabi promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mWasabi

Expression

Bacterial

Promoter

(None)

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCF709

Plasmid

#121891

Purpose

EF1a-ZIKV-protease-mTagBFP2

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Inserts

Use

Lentiviral

Promoter

EF1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG050

Plasmid

#113999

Purpose

SC module. Thermostable beta-galactosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG083

Plasmid

#114001

Purpose

SC module. Beta-glucosidase reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSG06-pLac-NRI

Plasmid

#102463

Purpose

Expresses NRI protein in E. coli. The expression is controlled by a pLac promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 122606

Insert

NRI (ntrC )

Expression

Bacterial

Promoter

pLac

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDTN3997

Plasmid

#113337

Purpose

Multi-copy 2-pyrone synthase plasmid for triacetic acid lactone production in Yarrowia lipolytica

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

multi-copy 2-pyrone synthase

Expression

Yeast

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_TEC-1/1

Plasmid

#91252

Purpose

Protein expression and purification of human SH3 domain construct TEC-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TEC-1/1 (TEC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP16

Plasmid

#80670

Purpose

Encodes the synthetic coiled coil peptide SYNZIP16

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP16

Use

Gateway Cloning

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pZa-tetO-csgAY-CmR

Plasmid

#98938

Purpose

Conductive Curli Protein csgAY

Depositor

Urartu Seker

Article

Kalyoncu et al RSC Adv. 2017, Issue 52. doi: 10.1039/C7

Insert

csgAY

Tags

YYRKYKEYDDY

Expression

Bacterial

Promoter

tetO

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Tags

6x Histidine

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP20

Plasmid

#80674

Purpose

Encodes the synthetic coiled coil peptide SYNZIP20

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP20

Use

Gateway Cloning

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Farnesylated SE pcDNA3.1

Plasmid

#79666

Purpose

Expresses super ecliptic A227D in mammalian cell membranes

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

superEcliptic pHluorin

Expression

Mammalian

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR-PLCE1i1

Plasmid

#59628

Purpose

Expression of shRNA against human PLCE1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PLCE1 (PLCE1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-PUb-SSA

Plasmid

#52910

Purpose

Ae aegypti PUb promoter Single Stranded Annealing reporter

Depositor

Zach Adelman

Article

Aryan et al PLoS One. 2013;8(3):e60082. doi: 10.1371

Insert

Luciferase duplication-TALEN recognition sites

Use

Luciferase

Promoter

Ae aegypti polyubiquitin

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGs-Hoxc8

Plasmid

#45555

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc8 (HOXC8 Chicken)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
PL06008A2C06 (smedwi2)

Plasmid

#35698

Depositor

Phillip Newmark

Article

Zayas et al Proc Natl Acad Sci U S A. 2005 Dec 20;10

Insert

Smedwi2

Promoter

T7 sense, T3 antisense

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only