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Showing: 901 - 920 of 19120 results

  1. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Article
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Use
    Tags
    T2A GFP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

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  2. pRS416-Gal4-dCas9-VP64

    Plasmid
    #71128
    Purpose
    This plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.
    Depositor
    Ronald Davis
    Article
    Naranjo et al PLoS Genet. 2015 Dec 29;11(12):e1005751.
    Insert
    Gal4-dCas9-VP64
    Use
    Tags
    NLS n-terminal, N terminal Gal4 Activator domain,…
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71128-plasmid-map-sequence-id-127063

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  5. pGL3_MSR1_bothneg0topos3pos1

    Plasmid
    #232387
    Purpose
    MSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox and right buffering Coordinator to sensitizing Coordinator
    Depositor
    Joanna Wysocka
    Insert
    MSR1 (MSR1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    MSR1 enhancer, left buffering Coordinator convert…
    Promoter
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232387-plasmid-map-sequence-id-462867

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  15. pCHC005 (∆RE)

    Plasmid
    #226199
    Purpose
    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226199-plasmid-map-sequence-id-453519

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  16. pGal4x5-E4T-G210

    Plasmid
    #171077
    Purpose
    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Insert
    Gal4-E4-Gless-cassette
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171077-plasmid-map-sequence-id-335554

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  18. Salsa6f

    Plasmid
    #140188
    Purpose
    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)
    Depositor
    Michael Cahalan
    Article
    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1
    Insert
    Salsa6f
    Use
    Tags
    tdTomato-v5-GCaMP6f
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Immediate Early
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140188-plasmid-map-sequence-id-316791

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Showing: 901 - 920 of 19120 results