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pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79621

Purpose

Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1

Expression

Mammalian

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pCMV

Available since

Sept. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)

Plasmid

#47430

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-SOScat

Plasmid

#178871

Purpose

Mammalian expression of SOS1(Rem-Cdc25) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-SOS1(Rem-Cdc25) (SOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA-Vav2cat

Plasmid

#178870

Purpose

Mammalian expression of Vav2(DH-PH-CRD) fused to PB1-AzamiGreen-FRB-TEVcs-mTagBFP2-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA-Vav2(DH-PH-CRD) (Vav2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Tagged Clavesin2 (pCMV-Tag2B-Sec14b)

Plasmid

#47431

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin2 (CLVS2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

Plasmid

#208379

Purpose

Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171615

Purpose

Soma-targeted Cal-Light with KA2

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171616

Purpose

Soma-targeted Cal-Light with Kv2.1

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

Plasmid

#209712

Purpose

Mammailan expression of WDR5, SH2, and HRas separated by P2A.

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

WDR5-P2A-SH2-P2A-Ras-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-(39PTC)

Plasmid

#184394

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein with PTC in beta-globin

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169591

Purpose

Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP and iRFP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

Plasmid

#169592

Purpose

Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

phloretin-controlled SEAP and iRFP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TtgO2-PCMVmin-2

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Use

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

Plasmid

#201545

Purpose

Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks N-terminal residues 1-79

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

Plasmid

#201548

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 364-409

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

Plasmid

#208378

Purpose

Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_WT aa 1-317 RAD23A (delta UBA2 domain)

Plasmid

#201465

Purpose

Expresses a mutant form of human RAD23A that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23A.

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 318-363

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge