We narrowed to 16,131 results for: form

1. p5E-gfap

   Plasmid

   #75024

   Purpose

   Can be used to drive expression in zebrafish astrocytes.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   glial fibrillary acidic protein promoter (gfap Zebrafish)
   

   Use

   5' entry vector for multisite gateway three …

   Promoter

   gfap

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. LTBP1-bio-His

   Plasmid

   #53421

   Purpose

   Expresses full-length Latent-transforming growth factor beta-binding protein 1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LTBP1 (LTBP1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

   Plasmid

   #229795

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 (-) hYAP 1-2alpha

   Plasmid

   #124143

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 (-) hYAP 1-2delta

   Plasmid

   #124146

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TrpC5 scFv [N67/15]

   Plasmid

   #190564

   Purpose

   Mammalian Expression of TrpC5 scFV. Derived from hybridoma N67/15.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   TrpC5 (Homo sapiens) recombinant scFV (TRPC5 Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Negative Control Binder

   Plasmid

   #179140

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9. Addi…

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3/hArf6(Q67L)-HA

   Plasmid

   #79425

   Purpose

   Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Glutamine 67 to Leucine

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/hArf6(T27N)-HA

   Plasmid

   #79426

   Purpose

   Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

   Insert

   Arf6 (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Threonine 27 to Asparagine

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Psicheck Cdk2ap1Exon1

    Plasmid

    #178028

    Purpose

    expression vector to test translation efficiency by luciferase reporter.

    Depositor

    Lin He

    Article

    Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

    Insert

    Cdk2ap1 (Cdk2ap1 Mouse)
    

    Use

    Luciferase; Nonviral

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    Cloned in

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/hArf1(WT)-mCherry

    Plasmid

    #79419

    Purpose

    Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf1 (ARF1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PGK Puro GFP-LC3B G120

    Plasmid

    #123241

    Purpose

    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    PGK

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3/hArf6(WT)-mCherry

    Plasmid

    #79422

    Purpose

    Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

    Plasmid

    #160566

    Purpose

    GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Edit (E3-E4-En-1)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid

    #190236

    Purpose

    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…

    Expression

    Mammalian

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. VGlut2 scFv [N29/29]

    Plasmid

    #182085

    Purpose

    Mammalian Expression of VGlut2 scFv. Derived from hybridoma N29/29.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting VGlut2 (Rattus norvegicus) (Slc17a6 Mouse)
    

    Tags

    HA, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. G protein alpha-q-YFP

    Plasmid

    #55782

    Purpose

    This G protein alpha-q construct contains internal insertions of YFP and the EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    alpha-q EE YFP (Gnaq Mouse)
    

    Tags

    The EE epitope was introduced into the alpha-q se…

    Expression

    Mammalian

    Mutation

    BamHI and SacI sites in alpha-q were removed with…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRES2-EGFP-deltaNp53:WTp53

    Plasmid

    #49244

    Purpose

    expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

    Depositor

    Dylan Taatjes

    Article

    Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

    Insert

    p53 deltaN and p53 WT (TP53 Human)
    

    Tags

    His and IRES-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR SFFVp BFP-Erk D319N

    Plasmid

    #102350

    Purpose

    Lentiviral expressing translational fusion between blue fluorescent protein and Erk2 bearing the D319N mutation

    Depositor

    Jared Toettcher

    Article

    Wilson et al Mol Cell. 2017 Sep 7;67(5):757-769.e5. d

    Insert

    Erk2 (Mapk1 Mouse)
    

    Use

    Lentiviral

    Tags

    BFP

    Mutation

    Aspartic acid 319 was mutated to asparagine

    Promoter

    SFFV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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