We narrowed to 18,860 results for: REV

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pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

Plasmid

#223287

Purpose

A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS

Use

Synthetic Biology; Piggybac transposase insertabl…

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-AMPKAR

Plasmid

#192449

Purpose

ExRai-AMPKAR targeted to the lysosomal membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Lyso-ExRai-AMPKAR

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEBTetD-β-tubulin_SNAP

Plasmid

#136832

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Expression

Bacterial

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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TL026_AAV_hSyn:SEP-GluA1

Plasmid

#192519

Purpose

Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

SEP-GluA1

Use

AAV and Cre/Lox

Tags

super ecliptic phluorin

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pJOG1368

Plasmid

#167301

Purpose

Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Insert

FCY-UPP fusion gene

Use

Synthetic Biology; Moclo level 0 vector

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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HypoxCR

Plasmid

#59946

Purpose

A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.

Depositor

Anne Le

Article

Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p

Inserts

GFP-Pest
mCherry-Geminin

Use

Lentiviral

Tags

FLAG and PEST

Expression

Mammalian

Promoter

CMV and min CMV

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAT9658-sgRNA-mCherry

Plasmid

#162987

Purpose

sgRNA cloning plasmid

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

exchangeable cassette

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-OSH2-2xPH

Plasmid

#161987

Purpose

Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

Insert

OSH2 (OSH2 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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LZF1827 pAAV_hSyn-Flp-off-Cre-on-stGtACR2-CFP

Plasmid

#135413

Purpose

expresses GtACR2 linked to CFP

Depositor

Adam Cohen

Article

Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

Insert

stGtACR2-CFP

Use

AAV

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCE27

Plasmid

#174405

Purpose

C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-ExRai-AMPKAR

Plasmid

#192448

Purpose

ExRai-AMPKAR targeted to the mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

Mito-ExRai-AMPKAR

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCE35

Plasmid

#174409

Purpose

C. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.

Depositor

Clarissa Nobile

Article

Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

Insert

C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12j3 VPR

Plasmid

#188517

Purpose

Expresses FLAG tagged dCas12j3 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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AMPK-γ1-HA

Plasmid

#40605

Depositor

Harvey Lodish

Article

Wong et al J Biol Chem. 2006 Nov 24;281(47):36434-4

Insert

AMPK-γ1 (Prkag1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
p-AAV-sh[SNCA]

Plasmid

#75437

Purpose

knockdown alpha-synuclein in rat neurons

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

snca (Snca Rat)

Use

AAV and RNAi

Expression

Mammalian

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA

Plasmid

#220221

Purpose

AAV-STARR-seq validation vector with GFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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