We narrowed to 17,817 results for: Met;

1. pEAHISMRSA2676

   Plasmid

   #96970

   Purpose

   expression of methicillin-resistant Staphylococcus aureus orf 2676

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Sar2676
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEAHISMRSAMVAK

   Plasmid

   #97000

   Purpose

   expression of methicillin-resistant Staphylococcus aureus MVAK protein

   Depositor

   James Naismith, Huanting Liu

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   Mevalonate kinase
   

   Use

   Tags

   N-ter TEV protease cleavable 6His

   Expression

   Mutation

   none

   Promoter

   T7

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

   Plasmid

   #129053

   Purpose

   targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA1 (mouseTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

   Plasmid

   #129029

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA1 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.2/V5-DEST CDC42EP1 S192A

   Plasmid

   #69820

   Purpose

   Expresses CDC42EP1-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   CDC42 effector protein (CDC42EP1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   S192A

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKAsub(TA)-TagRFPT-CAAX

   Plasmid

   #87740

   Purpose

   Negative-control PKA substrate fragment for bimFLINC-AKAR, targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

   Insert

   PKAsub(TA)-TagRFPT-CAAX
   

   Use

   Tags

   6xHis, C-terminal targeting sequence from Kras, T…

   Expression

   Mammalian

   Mutation

   Target Thr residue in substrate domain mutated to…

   Promoter

   CMV

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

   Plasmid

   #85821

   Purpose

   iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   Zf Ubi::cMyc oDam_f_GFPoNLS
   

   Use

   Tol2 based vector

   Tags

   cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

   Expression

   Mutation

   L122A. Codon optimization compatible to work in M…

   Promoter

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. JA431

   Plasmid

   #49937

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD KL-2 (KLF4 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRSF_his6_sumo_ZIKV_NS5_MTase

   Plasmid

   #213529

   Purpose

   Expresses the MTase domain of NS5 of ZIKV

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   ZIKA virus NS5 methyltransferase domain
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   MTase domain aa 1-265

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-CMV-scFVDnmt3L_bGHpA

    Plasmid

    #177350

    Purpose

    AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    194–415 aa

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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