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pFETCh_HBP1

Plasmid

#86272

Purpose

Donor vector for 3' FLAG tag of human HBP1

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

HBP1 homology arms (HBP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HMG20A

Plasmid

#86271

Purpose

Donor vector for 3' FLAG tag of human HMG20A

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

HMG20A homology arms (HMG20A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZSCAN9

Plasmid

#86268

Purpose

Donor vector for 3' FLAG tag of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Insert

ZSCAN9 homology arms (ZNF193 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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CMJ159

Plasmid

#69525

Purpose

pTET HochAld1-20-pduD21-224- FLAG

Depositor

Danielle Tullman-Ercek

Publication

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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CMJ157

Plasmid

#69523

Purpose

pTET eutE1-20-pduD21-224-FLAG

Depositor

Danielle Tullman-Ercek

Publication

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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CMJ156

Plasmid

#69522

Purpose

pTET eutC1-20-pduD21-224-FLAG

Depositor

Danielle Tullman-Ercek

Publication

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

PduD from Salmonella (pduD )

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Ubl4A K48R

Plasmid

#61809

Purpose

expresses mutated Ubl4A with K48R, with a C-terminal FLAG tag

Depositor

Yihong Ye

Publication

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

UBL4A (UBL4A Human)

Tags

FLAG

Expression

Mammalian

Mutation

K48R

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only
pMS08

Plasmid

#230072

Purpose

Constitutive expression Promoter (RpoD sigma factor, BT_RS06635), integrates via IntN1; N-/C-terminal mScarlet-I fusion, ermF, C-terminal 3x-FLAG, R6K ori, RP4 oriT

Depositor

Sean Crosson

Publication

Schnizlein et al Microbiol Resour Announc. 2025 May 8;14(

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG and mScarlet-I

Expression

Bacterial

Promoter

RpoD (bt_1311/BT_RS06635)

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP in pDBEcR-pIND(SP1)-W/Bla

Plasmid

#201558

Purpose

Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

Depositor

Randy Poon

Publication

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR and EGFP

Use

Unspecified; Sleeping beauty transposon

Tags

DBEcR-myc and FLAG-EGFP

Expression

Mammalian

Promoter

EF1a and pIND(SP1)

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Publication

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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GP805

Plasmid

#164545

Purpose

Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

All 21 cytoplamic Lysines of Smoothened changed t…

Promoter

GgCryD1

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA + S580A POLI

Plasmid

#131241

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert Pol iota se…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA POLI

Plasmid

#131239

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

Depositor

Roger Woodgate

Publication

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert lysine resi…

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-FMW-attB-AthHen1

Plasmid

#104958

Purpose

Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 drivers

Depositor

Stefan Ameres

Publication

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Insert

Hen1 (HEN1 Mustard Weed)

Tags

FLAG Myc

Expression

Insect

Promoter

Hsp70 promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-T830E

Plasmid

#91996

Purpose

Expresses FLAG-HA-AGO2 (T830E)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

T830E

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S834E

Plasmid

#91998

Purpose

Expresses FLAG-HA-AGO2 (S834E)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S834E

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-S828A

Plasmid

#91982

Purpose

Expresses FLAG-HA-AGO2 (S828A)

Depositor

Joshua Mendell

Publication

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828A

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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