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dCAS9-VP64_GFP

Plasmid

#61422

Purpose

Expresses dCAS9-VP64 activator with 2A GFP

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Concentrated Lentiviral Prep

Insert

dCAS9(D10A,H840A)-VP64_2A_GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

EF1A

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJWV102-PL-dCas9

Plasmid

#85588

Purpose

Integrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9sp

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

dCas9sp

Use

CRISPR

Expression

Bacterial

Promoter

PLsp

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Expression

Bacterial

Promoter

Arabinose-inducible pBAD

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex-GFP

Plasmid

#197883

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-TOMM20-N-10

Plasmid

#55328

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TOMM20 (TOMM20 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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H2B-mScarlet3-S2

Plasmid

#246872

Purpose

Expression of mScarlet3-S2 fused with H2B. This construct is used for imaging, photobleaching and photochromic behavior measurements.

Depositor

Pingyong Xu

Article

Ding et al Nat Methods. 2025 Nov 26. doi: 10.1038/s

Insert

mScarlet3-S2

Tags

H2B

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk1a1

Plasmid

#13798

Depositor

Roger Davis

Article

Derijard et al Cell. 1994 Mar 25. 76(6):1025-37.

Insert

Jnk1a1 (MAPK8 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FlipGFP(Mpro) T2A mCherry

Plasmid

#163078

Purpose

Expresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry

Depositor

Xiaokun Shu

Article

Li et al PLoS Pathog. 2021 Sep 3;17(9):e1009898.

Insert

FlipGFP-based Mpro activity reporter and mCherry

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmBaoJin-NUP98

Plasmid

#229594

Purpose

Expresses mBaoJin-NUP98 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

Insert

mBaoJin

Expression

Mammalian

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pREDTAI

Plasmid

#51627

Purpose

E coli Genetic Modification

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Insert

I-SceI (SCEI Budding Yeast)

Use

Red recombinase and i-scei endonuclease

Expression

Bacterial

Promoter

trc

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas3

Plasmid

#209427

Purpose

Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa

Use

CRISPR and Synthetic Biology

Promoter

PtipA; crRNA under control of PermE*

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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N8his-GFPenhancer-GGGGS4-LaG16

Plasmid

#140442

Purpose

We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

Depositor

Motoyuki Hattori

Article

Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

Insert

GFPenhancer-GGGGS4-LaG16

Tags

8 histidine tag

Expression

Bacterial

Promoter

T7 promotor

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-PEmax-P2A-Puro

Plasmid

#228890

Purpose

Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

Depositor

Francisco J Sánchez-Rivera

Article

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Inserts

PEmax
Puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB502

Plasmid

#74844

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry3C(1-10)

Plasmid

#117482

Purpose

Lentiviral vector for constitutive expression of sfCherry3C(1-10)

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

sfCherry3C(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

5 substitutions (K45R, G52D, T106A, K182R, N194D)…

Promoter

SFFV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1delltaH5

Plasmid

#71714

Purpose

Membrane scaffold protein (MSP), helix 5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGreenLantern

Plasmid

#161912

Purpose

mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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