We narrowed to 13,184 results for: BASE

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pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

Plasmid

#160570

Purpose

A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

sgRNA:scaffold tetraloop MS2 aptamer SAM

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

Plasmid

#131757

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#2 Sp/xCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

Plasmid

#131756

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#1 Sp/xCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

Plasmid

#131755

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#1 SaCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

Plasmid

#131758

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#2 SaCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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1010-AIDx-CP

Plasmid

#135340

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

CP 1010 nSpyCas9 -hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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1058-AIDx-CP

Plasmid

#135342

Purpose

Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

CP 1058 nSpyCas9-hAIDx

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-208

Plasmid

#135345

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-208

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-468

Plasmid

#135346

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-468

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.-1

Plasmid

#133539

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 36 bp upstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.25

Plasmid

#133538

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 28 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pY71-tet.I.20

Plasmid

#133537

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 23 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.14

Plasmid

#133535

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 17 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.13

Plasmid

#133534

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 16 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.12

Plasmid

#133533

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 15 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.11

Plasmid

#133532

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 14 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.10

Plasmid

#133531

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 13 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.9

Plasmid

#133530

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 12 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.7

Plasmid

#133529

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 10 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pY71-tet.I.6

Plasmid

#133528

Purpose

sfGFP driven by a T7 promoter with the tetO sequence 9 bp downstream from the promoter start site

Depositor

Richard Murray

Article

McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

Insert

sfGFP

Tags

Strep tag and TEV site

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge