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NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1

Plasmid

#183404

Purpose

Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-GID-SmBiT-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1

Plasmid

#183399

Purpose

Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus

Depositor

Alice Ting

Article

Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

Insert

GI-VP16-IRES-GAL4DBD-NanoLuc-FKF1

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_SMO

Plasmid

#183321

Purpose

All-in-One CRISPRko system with a guide RNA that targets SMO gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

SMO

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (Pc Fly)

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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CcCDP_p15A

Plasmid

#179272

Purpose

Expresses CcCDP in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Insert

cellodextrin phosphorylase

Use

Synthetic Biology

Tags

6xHIS

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Hygro_zhang2.0

Plasmid

#167915

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EYFP_zhang2.0

Plasmid

#167914

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_KanR neo

Plasmid

#167893

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_iRFP713

Plasmid

#167892

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Hygro

Plasmid

#167891

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EBFP2

Plasmid

#167889

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Blasto

Plasmid

#167888

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_iRFP713

Plasmid

#167868

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_Hygro

Plasmid

#167867

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_EBFP2

Plasmid

#167865

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_iRFP713

Plasmid

#167860

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8_28bp

Plasmid

#176185

Purpose

plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI1up

Plasmid

#176166

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-1

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC7-XI1dw

Plasmid

#176167

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-1

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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