We narrowed to 9,895 results for: EPO

1. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2.86

   Plasmid

   #212538

   Purpose

   Encodes SARS-CoV-2 variant BA.2.86 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2.86 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220608

   Purpose

   Neuron-specific, Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

   Plasmid

   #179437

   Purpose

   Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   coreHS4-TRE-cit-coreHS4-mch (DC)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-Promoter-hPE(+)-Luc

   Plasmid

   #172003

   Purpose

   Luciferase Reporter for Human PTEN Enhancer PE - Forward Orientation

   Depositor

   Daniel Herranz

   Article

   Tottone et al Blood Cancer Discov. 2021 Jan;2(1):92-10

   Insert

   Human PE sequence (hPE)
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   SV40 Promoter

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDN-D2irTN6kwh

   Plasmid

   #44724

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   htetR::NLS
   

   Use

   Synthetic Biology; Expression regulator/reporter;…

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-ZIP(RK)

   Plasmid

   #27136

   Depositor

   Ron Vale

   Article

   Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

   Insert

   CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   eGFP and mCherry

   Expression

   Mammalian

   Mutation

   Arginines in the SH2 domains mutated to lysines (…

   Promoter

   Available Since

   April 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti_EF1a_Flag_TP53_P278A

   Plasmid

   #183113

   Purpose

   Expresses flag-tagged p53 with P278A mutation in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed proline 278 to alanine, see depositor com…

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p1.1-mCherry

    Plasmid

    #162772

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    red fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p1.2-GS-eGFP

    Plasmid

    #162771

    Purpose

    Fluorescent reporter for CHO expression studies

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

    Plasmid

    #159088

    Purpose

    LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Inserts

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    eGFP
    polyA
    

    Use

    Fluorescent reporter

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNIC-ZB-SPRTN-E112A-FL

    Plasmid

    #110218

    Purpose

    Expression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.

    Depositor

    Kristijan Ramadan

    Article

    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    His6 and ZB

    Expression

    Bacterial

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3 2198

    Plasmid

    #49347

    Purpose

    This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

    Depositor

    Melissa Rogers, Stephen Harris

    Article

    Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

    Insert

    Bmp2 promoter (shorter sequence) (BMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Bmp2 promoter

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tre-Tight ifgMosaic Donor vector (LH121)

    Plasmid

    #99630

    Purpose

    To clone the 3 ORFs for Tet system (rtTA + Dox) dependent generation of ifgMosaics

    Depositor

    Rui Benedito

    Article

    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

    Insert

    N-PhiM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    (Please see depositor comments below)

    Promoter

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMiR-RECK-3'UTR-miR-21-mut

    Plasmid

    #53693

    Purpose

    Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-21 binding site

    Depositor

    Edward Chan

    Article

    Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

    Insert

    RECK-3'UTR (RECK Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    nucleotide sequence 1083-1086 is mutated to TTCG

    Promoter

    Available Since

    July 11, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pGL2-hOct4 DE-SV40-Luc

    Plasmid

    #26279

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments below

    Promoter

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIS1 INSIG2

    Plasmid

    #60798

    Purpose

    Luciferase reporter to measure miR-155 mediated differential repression. Contains INSIG2 3' UTR and wild-type miR-155 sites

    Depositor

    David Bartel

    Article

    Nam et al Mol Cell. 2014 Mar 20;53(6):1031-43. doi

    Insert

    INSIG2 3'UTR and wild-type miR-155 binding site (INSIG2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase (HSV-TK promoter)

    Available Since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid

    #179429

    Purpose

    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-5kb-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge