159,909 results

1. pcDNA3-nuc-GsCT

   Plasmid

   #205517

   Purpose

   Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-GsCT (GNAS Synthetic, Human)
   

   Use

   Tags

   Histone H2A and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29b-H6_Streptavidin_sfGFP

   Plasmid

   #124296

   Purpose

   His6-tagged Streptavidin GFP fusion protein

   Depositor

   Mark Arbing

   Insert

   His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
   

   Use

   Tags

   His6 and T7 tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159857

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJ5 mCnB

   Plasmid

   #17872

   Depositor

   Jerry Crabtree

   Article

   Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

   Insert

   CnB (Ppp3r1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCXLE-SOX2-p2a-KLF4

   Plasmid

   #193292

   Purpose

   episomal hSK (expressing human SOX2 + KLF4)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2-p2a-KLF4 (SOX2, KLF4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F2A of original pCXLE-SK vector was replaced with…

   Promoter

   CAG

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. mRNA-CleanCap-NanoLuc

   Plasmid

   #188247

   Purpose

   Template plasmid to transcribe mRNA encoding NanoLuc

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   NanoLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-HA-UBE3BΔHECT-IRES-Puro

   Plasmid

   #107347

   Purpose

   Expresses HA tagged UBE3B deletion of HECT domain in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3BΔHECT (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deletion HECT domain (757-end)

   Promoter

   CMV

   Available since

   June 26, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. cfSGFP2-anti-AlfaTag nanobody

   Plasmid

   #171818

   Purpose

   Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

   Depositor

   Ikuo Wada

   Article

   Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

   Insert

   cfSGFP2-anti-AlfaTag nanobody
   

   Use

   Tags

   6xHis and SEP tag (6xD)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CHRNA7-YFP

    Plasmid

    #62630

    Purpose

    mammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    hAlpha7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    YFP inserted in M3-M4 loop

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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