We narrowed to 14,613 results for: sta

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pLVX-Flag-PolB(T304I)-Puro

Plasmid

#177143

Purpose

Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-CRY2oligo-mcherry-ANXA11(R346C)

Plasmid

#164206

Purpose

Expresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Tags

CRY2oligo-mcherry

Expression

Mammalian

Mutation

R346C

Promoter

CMV

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR10

Plasmid

#167003

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 10 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-GFP-IQSEC1 v2-E620K GEF dead mutant

Plasmid

#162020

Purpose

Expression of GEF dead IQSEC1 mutant

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

IQSEC1 (IQSEC1 Human)

Use

Lentiviral

Mutation

EK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ELF2_pet28a

Plasmid

#131662

Purpose

Bacterial expression of ETS gene ELF2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF2 (ELF2 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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JL302: pMVP/Puro-DEST

Plasmid

#121856

Purpose

pMVP destination vector, empty expression vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127316

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127318

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127321

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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GHR1 X017_pECIA14

Plasmid

#114847

Purpose

Prey vector GHR1 X017_pECIA14 should be used with bait vector GHR1 X017_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G20940 (GHR1 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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ERECTA C6_pECIA2

Plasmid

#115127

Purpose

Bait vector ERECTA C6_pECIA2 should be used with prey vector ERECTA C6_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G26330 (ER Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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HA-human-BNIP3 delta Exon2

Plasmid

#100782

Purpose

Mammalian expression of BNIP3 delta Exon2 splice variant

Depositor

Joseph Gordon

Article

Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

Insert

human-BNIP3 delta Exon2 (BNIP3 Human)

Tags

HA

Expression

Mammalian

Mutation

Exon2 deletion

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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tRNA-gRNA position [D1_2] (GB1205)

Plasmid

#75406

Purpose

tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_2]) of a polycistronic tRNA-gRNA regulated by the dicot U6-26 or U6-1 promoter (3-part multiplexing)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

tRNA-gRNA

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-XPLN aa452-526

Plasmid

#73381

Purpose

Expresses amino acids 452-526 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 452-526 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa304-466 (PH)

Plasmid

#73380

Purpose

Expresses amino acids 304-466 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 304-466 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Myc-XPLN aa107-311

Plasmid

#73379

Purpose

Expresses amino acids 107-317 of XPLN (Arhgef3) protein

Depositor

Jie Chen

Article

Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

Insert

arhgef3 (ARHGEF3 Human)

Tags

Myc (QKLISEEDL)

Expression

Mammalian

Mutation

Expresses amino acids 107-317 of XPLN (arhgef3) p…

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO-PBG-WPRE-hGH

Plasmid

#74287

Purpose

pAAV vector to express PBG (chimeric Glycoprotein) in a Cre-dependent manner

Depositor

Edward Callaway

Article

Kim et al Neuron. 2015 Dec 16;88(6):1253-67. doi:

Insert

PBG (chimeric rabies Glycoprotein)

Use

AAV

Promoter

Ef1a

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKG427

Plasmid

#69754

Purpose

Retroviral expression of DSN1

Depositor

Iain Cheeseman

Article

Gascoigne et al J Cell Biol. 2013 Apr 1;201(1):23-32. do

Insert

DSN1 (DSN1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

all K to R mutations

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only
2.3 hA33 luciferase

Plasmid

#68148

Purpose

luciferase reporter for hA33 promoter

Depositor

Joan Heath

Article

Johnstone et al J Biol Chem. 2002 Sep 13;277(37):34531-9

Insert

A33 (GPA33 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

starts at -2270 relative to ATG

Promoter

human GPA33 promoter

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only