162,536 results

1. 7i sgRNA for 4-μHOM reporter

   Plasmid

   #113626

   Purpose

   sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7i sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pLNCX2-mCherry-CHMP4B

   Plasmid

   #116923

   Purpose

   expresses mCherry-CHMP4B in mammalian cells

   Depositor

   Sanford Simon

   Article

   Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

   Insert

   CHMP4B (CHMP4B Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-Sox2

   Plasmid

   #16353

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xnls-mNeonGreen

   Plasmid

   #98875

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv 160374

   Insert

   3xNLS
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry-PCNA

   Plasmid

   #215117

   Purpose

   Expresses mCherry-PCNA in mammalian cells from a lentiviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-tet-iCas9-BFP2

   Plasmid

   #125519

   Purpose

   all-in-one-inducible Cas9

   Depositor

   Jason Moffat

   Article

   Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

   Insert

   AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

    Plasmid

    #224435

    Purpose

    AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mScarlet-KASH
    U6-gRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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