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pILAC3

Plasmid

#185890

Purpose

In vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locus

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96C; Xd.CRtYB E83K

Available since

Sept. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1-PPPR2D-2

Plasmid

#179355

Purpose

shRNA suppression

Depositor

William Hahn

Publication

Sablina et al Cancer Res. 2010 Dec 15;70(24):10474-84.

Insert

B55δ

Use

Lentiviral

Expression

Mammalian

Promoter

Age1

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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PROM5_35Sx2

Plasmid

#136112

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35Sx2 (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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3TERM_Nos

Plasmid

#136115

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_Nos (A. tumefaciens)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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PROM5_35S

Plasmid

#136111

Purpose

Strong promoter that drives expression everywhere in Mp gemmae but much weaker in the notches

Depositor

Jim Haseloff

Publication

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-TREtight-H2b-emiRFP670-P2A-TEVP

Plasmid

#174379

Purpose

AAV virus for expressing TEVP and H2b-emiRFP670

Depositor

Ian Wickersham

Publication

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TEVP

Use

AAV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_OctaHis-GFP11

Plasmid

#153509

Purpose

OctaHis-GFP11 C-terminal Tag Donor Plasmid

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

OctaHis-GFP11

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-TTLL11

Plasmid

#140582

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Publication

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA TTLL11

Use

CRISPR

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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T7-AglK-His

Plasmid

#89714

Purpose

Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

Depositor

Barbara Imperiali

Publication

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

AglK

Tags

His6 and T7

Expression

Bacterial

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Publication

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Mutation

AT-swapped mutant

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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p3E NWASP-CA

Plasmid

#105968

Purpose

3' element for Gateway cloning, NWASP C terminal domain

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NWASP-CA

Expression

Bacterial

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJG367

Plasmid

#91185

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_H840A+gNt_R2+gNt_F2+donor

Use

CRISPR

Expression

Plant

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJG382

Plasmid

#91189

Purpose

BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nAtCas9_D10A+gNt_R2+gNt_F2+donor

Use

CRISPR

Expression

Plant

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pARiBo1

Plasmid

#98892

Purpose

Expresses ARiBo-fused RNA in vitro with T7 RNA polymerase

Depositor

Pascale Legault

Publication

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

ARiBo1

Use

In vitro transcription vector

Promoter

T7 promoter

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Publication

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
4IGZ

Plasmid

#51323

Depositor

Cheryl Arrowsmith

Insert

4IGZ (SORBS2 Human)

Tags

His

Expression

Bacterial

Mutation

Truncation: See Comments

Promoter

T7

Available since

Feb. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
pCMV-Rab-Rep

Plasmid

#45967

Depositor

Ralf Kuehn

Insert

beta-Galactosidase

Expression

Mammalian

Mutation

duplicated aa 1-135

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P36

Plasmid

#46007

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P36 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pSA25

Plasmid

#35116

Depositor

John McCusker

Publication

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5MX4

Use

Yeast replicating

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBN62

Plasmid

#35105

Depositor

John McCusker

Publication

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1PR

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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