We narrowed to 3,259 results for: Ubc

1. CyPet-FKBP-E2

   Plasmid

   #175244

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJR2953

   Plasmid

   #122549

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemCardinal2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pnhx-2

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. optoPKAW196R/F327A

   Plasmid

   #104547

   Purpose

   Expresses Cry2-mCherry-PKA W196R/F327A in mammalian cells.

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Cry2-mCherry-PKA W196R/F327A
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   PKA W196R and Y204A

   Promoter

   CMV

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CALNL-DCX-eGFP

   Plasmid

   #28310

   Depositor

   Joseph Loturco

   Article

   Manent et al Nat Med. 2009 Jan . 15(1):84-90.

   Insert

   Doublecortin (Dcx Rat)
   

   Use

   Cre/Lox

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2011

   Availability

   Academic Institutions and Nonprofits only

5. ZE43 Dapper-2 pCS2P+

   Plasmid

   #17084

   Depositor

   Randall Moon

   Article

   Waxman et al Development. 2004 Dec. 131(23):5909-21.

   Insert

   Dapper-2 (dact2 Zebrafish)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pMP650

   Plasmid

   #135000

   Purpose

   VP64-m6A Reader. Activates transcription near Gm6ATC sites. pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR

   Depositor

   Ahmad Khalil

   Article

   Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.

   Insert

   pUBC-DpnI(aa146-254)-VP64-V5 pGK-ZeoR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   DpnI(aa146-254), V5 tag, and VP-64

   Expression

   Mammalian

   Mutation

   DpnI truncated to aa146-254

   Promoter

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YFP(1-158) in pcDNAI/Amp

   Plasmid

   #60888

   Purpose

   This vector attaches YFP(1-158) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(1-158)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met was substituted for Gln-69.

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YFP(159-238) in pcDNAI/Amp

   Plasmid

   #60889

   Purpose

   This vector attaches YFP(159-238) to the N-terminus of a protein.

   Depositor

   Catherine Berlot

   Article

   Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

   Insert

   YFP(159-238)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPMEL-Nluc-hbb

   Plasmid

   #239862

   Purpose

   Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.

   Depositor

   Catherine Jopling

   Article

   Smart et al Molecular Therapy Nucleic Acids, 102602.

   Insert

   Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   T7 (included as part of insert)

   Available since

   July 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid

    #236097

    Purpose

    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

    Depositor

    Jin Zhang

    Article

    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

    Insert

    ExRai-CKAR2-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHD-FlpTag-DsRed-HDR-ph2

    Plasmid

    #231902

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 2.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHD-FlpTag-DsRed-HDR-ph1

    Plasmid

    #231901

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 1.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHD-FlpTag-DsRed-HDR-ph0

    Plasmid

    #231900

    Purpose

    dsDNA donor vector with 3xP3-DsRed and FlpTag-GFP for precise genomic targeting and integration of the FlpTag-GFP cassette with codon phase 0.

    Depositor

    Alexander Borst

    Article

    Fendl et al Elife. 2020 Oct 20;9:e62953. doi: 10.755

    Insert

    Frt-Frt14-SD-GFP-SA-Frt-Frt14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGEN_N73_eMagBF_V5

    Plasmid

    #235642

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5

    Expression

    Mammalian

    Mutation

    residues 1-73

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGEN_Fbl_N73_eMagBF_V5

    Plasmid

    #235643

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, Fbl for targeting to nucleolus.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    Fbl-N73- and eMagBF-V5

    Expression

    Mammalian

    Mutation

    residues 1-73

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX459_L31_CRISPR_downstream_sgRNA

    Plasmid

    #235610

    Purpose

    Encodes CRISPR gRNA for cleaving downstream of insertion site at C terminus of mouse Rpl31

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    Rpl31 C-terminus downstream sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX459_L31_CRISPR_upstream_sgRNA

    Plasmid

    #235609

    Purpose

    Encodes CRISPR gRNA for cleaving upstream of insertion site at C terminus of mouse Rpl31

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    Rpl31 C-terminus upstream sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGEN_N73_eMagBF_V5_NES

    Plasmid

    #235614

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-73

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGEN_NES_V5_BirA

    Plasmid

    #235634

    Purpose

    Encodes full-length BirA fused to a nuclear export sequence and V5. Used as a positive control in ALIBi experiments

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    BirA
    

    Use

    Tags

    NES-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGEN_N65_eMagBF_V5_NES

    Plasmid

    #235612

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-65, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-65

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCAGEN_N77_eMagBF_V5_NES

    Plasmid

    #235616

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-77, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-77

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGEN_N99_eMagBF_V5_NES

    Plasmid

    #235618

    Purpose

    Encodes ALIBi N-terminal fragment, residues 1-99, fused to eMagBF (enhanced Magnet, basic, fast), V5, and nuclear export sequence.

    Depositor

    Maria Barna

    Article

    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

    Insert

    ALIBi N-terminal fragment
    

    Use

    Tags

    eMagBF-V5-NES

    Expression

    Mammalian

    Mutation

    residues 1-99

    Promoter

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Esyn-FlpX-TVA950-EYFP-WPRE

    Plasmid

    #234710

    Purpose

    pAAV transfer plasmid with enhanced Synapsin promoter driving Flp-dependent expression of TVA950-eYFP

    Depositor

    David Ng

    Article

    Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

    Insert

    TVA950-eYFP
    

    Use

    AAV; Has fdio cassette for flp-dependent expressi…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    enhanced Synapsin

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225062

    Purpose

    Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

    Plasmid

    #225061

    Purpose

    Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

    Plasmid

    #225063

    Purpose

    Expression of CylM-modified CylLL-S15T in human cells

    Depositor

    Wilfred van der Donk

    Article

    Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

    Insert

    CylLL-S15T
    

    Use

    Tags

    Multiple tags due to polycistronic expression

    Expression

    Mammalian

    Mutation

    S15T

    Promoter

    CMV

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX-PML RB1

    Plasmid

    #185636

    Purpose

    expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells

    Depositor

    Irina Bezsonova

    Article

    Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

    Insert

    promyelocytic leukemia protein (PML Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-C1-IFT22

    Plasmid

    #218720

    Purpose

    Expresses N-terminally EGFP-tagged IFT22 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

    Insert

    IFT22 (RABL5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MCP-mCherry-TIS11B

    Plasmid

    #207670

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    TIS11B (ZFP36L1 Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3-HA PSPC1 Y523F

    Plasmid

    #124373

    Purpose

    Expresses PSPC1 Y523F in mammalian cells

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 (PSPC1 Human)
    

    Use

    Tags

    HA and HA tag

    Expression

    Mammalian

    Mutation

    Y523F

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge