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Plasmid#173143PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj13 (kcnj13 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj15
Plasmid#173145PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj15 (kcnj15 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj3a
Plasmid#173129PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj3a (kcnj3a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj5
Plasmid#173132PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj5 (kcnj5 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj6
Plasmid#173133PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj6 (kcnj6 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFendoNA2S
Plasmid#174730PurposeExpresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.DepositorInsertendoNA2-S706A
UseTagsExpressionBacterialMutationChanged Serine 706 to AlaninePromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHBS1506 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-L]
Plasmid#133330PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll FYW to L in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1507 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-S]
Plasmid#133331PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1508 [IBB-GFP-mCherry 3E]-[BFP-TDP43 VLIM-F]
Plasmid#133332PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43PromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1397 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 W385G]
Plasmid#118812PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationW385G in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1399 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G368W+G385W]
Plasmid#118813PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG368W and W385G in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1400 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 G295F]
Plasmid#118814PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationG295F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1393 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaRRM]
Plasmid#118805PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of RRM in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1394 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaCTD]
Plasmid#118806PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of CTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]
Plasmid#118807PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationDeletion of NTD in full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1401 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 F-S]
Plasmid#118808PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll F to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1404 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-S]
Plasmid#118809PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to S in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1405 [IBB-GFP-mCherry 3E]-[BFP-P2A-TDP43 VLIM-F]
Plasmid#118810PurposeTo test the effect of sequence on TDP43 splicing activityDepositorInsertTARDBP mutant (TARDBP Human)
UseTagsExpressionMammalianMutationAll VLIM to F in CTD of full-length TDP43PromoterAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPICZ:hTRAAK(W262S)
Plasmid#130670PurposeP. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFPDepositorInsertKCNK4 (KCNK4 Human)
UseTagsSNS- 3C_cleavage_site-TAAA-GFPExpressionYeastMutationN104Q, N108Q, W262SPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pIRES:hTRAAK(W262S)
Plasmid#133078PurposeMammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutantDepositorInsertPotassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pIRES:hTRAAK(G124I)
Plasmid#133079PurposeMammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutantDepositorInsertPotassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
UseTagsExpressionMammalianMutationG124IPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-G9aCD
Plasmid#235580PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector G9a fused with scFV to programme H3K9me2/3DepositorInsertG9a (Ehmt2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2
Plasmid#221225PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporterDepositorInsertAID-PcrA M6-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ezh2FL
Plasmid#235575PurposeDox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3DepositorInsertEzh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFR G719S
Plasmid#11013PurposeRetroviral construct for expressing human EGFR with G719S mutation in human cellsDepositorInsertEGFR G719S (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationG719SPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTargetF-FRT
Plasmid#113637PurposeDerivative of pTargetF with gRNA targeting FRT siteDepositorInsertsgRNA-FRT
UseCRISPRTagsExpressionMutationPromoterpJ23119Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFR L858R
Plasmid#11012PurposeRetroviral construct for expressing human EGFR with L858R mutation in human cellsDepositorInsertEGFR L858R (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationL858R (tumor-derived)PromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-BLM-UGI-T2A-mTagBFP2
Plasmid#221222PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporterDepositorInsertAID-BLM-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ezh2FL-CatMut
Plasmid#235584PurposeDox-inducible expression of control catalytic-mutant Ezh2 fused with scFVDepositorInsertEzh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMutationY726DPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-tomato SOX2-sgRNA
Plasmid#196190PurposeEditing human SOX2 locusDepositorInsertSOX2 (SOX2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Dot1LCD
Plasmid#235582PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dot1l fused with scFV to programme H3K79me2DepositorInsertDot1l (Dot1l Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-PcrA-UGI-T2A-mTagBFP2
Plasmid#221224PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporterDepositorInsertAID-PcrA-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-AID-Ns3h-UGI-T2A-mTagBFP2
Plasmid#221223PurposeExpress HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase with mTagBFP2 reporterDepositorInsertAID-Ns3h-UGI-T2A-mTagBFP2
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTP261
Plasmid#104166PurposeBacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation1x Cysteine located at bps 493-495 in full sequen…PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-XBP1u
Plasmid#63679PurposeAdenoviral shuttle vector for XBP1u expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationunsplicedPromoterCMVAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorInsertEGFR D837A (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationD837APromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
SBC015866
Plasmid#226280PurposeExpresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.DepositorInserts4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MultiMate 5 colors
Plasmid#206266PurposeExpression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorInsertH2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
UseRecombinant baculovirus production, cre acceptor…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
UseTagsExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
H14_MBP_SUMO-Cys-linker-BAF
Plasmid#101777PurposeExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationPromoterT5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs T751A
Plasmid#107142Purposefull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vectorDepositorInsertSSeCKs T751A (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationchanged threonine 751 to alaninePromotergagAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only