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pCDH_CMV_HMGB_ctail_mKate2_CLEAVAGE

Plasmid

#167338

Purpose

Expresses mKate2 fused to TFAM HMGB+C-tail mutant in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) as well a…

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSG2_p.S265C

Plasmid

#81338

Purpose

Gateway Donor vector containing PSG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PSG2 (PSG2 Human)

Use

Gateway entry vector

Mutation

V20L; S265C; p.333_335delNPT-insIQH

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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ADCK2 gRNA (BRDN0001145856)

Plasmid

#77338

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK2 (ADCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-HIV RT-6xHis-RBS-prot

Plasmid

#159149

Purpose

expresses His-tagged HIV reverse transcriptase and untagged HIV protease

Depositor

Andrea Pauli

Article

Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

Inserts

Human immunodeficiency virus 1 (HIV-1) reverse transcriptase
Human immunodeficiency virus 1 (HIV-1) protease

Tags

His

Expression

Bacterial

Mutation

HIV-1 group M/subtype B – BH10 strain

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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His-GST-TEV-BRD4(42-459) BD1-BD2

Plasmid

#233887

Purpose

pGex for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

His-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVLINK-EF1alpha-HA-5'-KIF14

Plasmid

#244338

Purpose

5' AAVLINK plasmid for KIF14 expression

Depositor

Zhonghua Lu

Article

Lin et al Cell. 2026 Feb 5;189(3):969-986.e17. doi

Insert

KIF14 (KIF14 Human)

Use

AAV

Tags

HA

Promoter

EF1alpha

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGluSnFR3.v857.GPI

Plasmid

#178338

Purpose

Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV GFAP iGluSnFR3 v857.GPI

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

GPI Anchor, IgK-chain, and Myc epi tag

Expression

Mammalian

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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p-Tet-O-RUNX1T1-Puro

Plasmid

#221666

Purpose

Expresses RUNX1T1 in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

RUNX1T1 (RUNX1T1 Human)

Use

Lentiviral

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC6A4_STOP

Plasmid

#161338

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A4 (SLC6A4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PKDCC

Plasmid

#23387

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKDCC (PKDCC Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PHKG1

Plasmid

#23385

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKG1 (PHKG1 Human)

Use

Gateway donor vector

Mutation

C556T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKX

Plasmid

#23383

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKX (PRKX Human)

Use

Gateway donor vector

Mutation

V43A

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
atp2c1_L (OZ567)

Plasmid

#33381

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting atp2c1 (atp2c1 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only
dio2_L (OZ569)

Plasmid

#33383

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting dio2 (dio2 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
dio2_R (OZ570)

Plasmid

#33384

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting dio2 (dio2 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
HACE1_R (OZ566)

Plasmid

#33380

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting HACE1 (LOC572430 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
atp2c1_R (OZ568)

Plasmid

#33382

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting atp2c1 (atp2c1 Zebrafish)

Use

Zebrafish targeting

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
p-Tet-O-EYA2-Puro

Plasmid

#221669

Purpose

Expresses EYA2 in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

EYA2 (EYA2 Human)

Use

Lentiviral

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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p-Tet-O-Foxp1A-Puro

Plasmid

#221665

Purpose

Expresses Foxp1A in mammalian cells

Depositor

Vanessa Hall

Article

Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

Insert

Foxp1A (Foxp1 Mouse)

Use

Lentiviral

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO_S.V5.NWS_NGFR

Plasmid

#158338

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

S.V5.NWS

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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