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Showing: 921 - 940 of 12274 results

  2. pCB-1

    Plasmid
    #210387
    Purpose
    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces
    Depositor
    Gilles van Wezel
    Article
    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021
    Inserts
    dCas9
    CymR
    sgRNA cassette
    Use
    CRISPR; Integrative vector for use in streptomyces
    Tags
    Expression
    Mutation
    BsaI-flanked RFP cassette inserted in place of sp…
    Promoter
    SP11 and SP30
    Available since
    Jan. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    210387-plasmid-map-sequence-id-418472

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  7. VinTS-tCRMod-GGSGGS9

    Plasmid
    #111765
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111765-plasmid-map-sequence-id-214720

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  11. pEditC

    Plasmid
    #207532
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207532-plasmid-map-sequence-id-409609

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  12. pEditA-RY

    Plasmid
    #207533
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207533-plasmid-map-sequence-id-409612

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  13. pEditC-RY

    Plasmid
    #207534
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207534-plasmid-map-sequence-id-409613

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  14. VinTS-tCRMod-GGSGGS7

    Plasmid
    #111764
    Purpose
    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 module
    Depositor
    Brenton Hoffman
    Article
    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1
    Insert
    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Residues 1-228 of Clover, residues 1-3 deleted fr…
    Promoter
    CMV
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111764-plasmid-map-sequence-id-214718

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Showing: 921 - 940 of 12274 results