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Showing: 921 - 940 of 26021 results

  7. pSLC-242

    Plasmid
    #73189
    Purpose
    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    chloramphenicol promoter
    Available since
    July 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    73189-plasmid-map-sequence-id-153071

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  8. pRedCm

    Plasmid
    #176225
    Purpose
    LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176225-plasmid-map-sequence-id-347001

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  9. SG3ΔENV RT D185A

    Plasmid
    #145782
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Reverse Transcriptase
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Reverse Transcriptase Aspartic Acid 185 t…
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    145782-plasmid-map-sequence-id-352197

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  12. SG3ΔENV CA E28A,E29A

    Plasmid
    #145785
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residues Glutamic Acid residues 28…
    Promoter
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    145785-plasmid-map-sequence-id-313273

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  13. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PPhlF
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  14. SG3ΔENV CA Q63A,Q67A

    Plasmid
    #145784
    Purpose
    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).
    Depositor
    Wesley Sundquist
    Article
    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/
    Insert
    Capsid
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Changed Capsid residues Glutamines 63 and 67 to a…
    Promoter
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    145784-plasmid-map-sequence-id-316752

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  16. pBP-T7_RBS-His6-Thrombin

    Plasmid
    #72979
    Purpose
    T7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    T7+RBS+His6+thrombin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  19. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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Showing: 921 - 940 of 26021 results