We narrowed to 4,390 results for: NAP

1. pET SUMO-syt1 (96-421) I367C

   Plasmid

   #213620

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 (96-421) I367C (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   C277A; I367C

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET SUMO-syt7 (136-403)

   Plasmid

   #213621

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 (136-403)

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 (136-403) (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET SUMO-syt1 5W

   Plasmid

   #213623

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET SUMO-syt1 s7+

   Plasmid

   #213624

   Purpose

   Bacterial expression of SUMO-tagged rat syt1 s7+

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt1 s7+ (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET SUMO-syt7 C2AB - 4A

   Plasmid

   #213625

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB - 4A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB - 4A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   F167A, F229A, I298A, L361A

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET SUMO-syt7 C2A-2A 2B

   Plasmid

   #213626

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2A-2A 2B (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   F167A, F229A, R363H

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET SUMO-syt7 C2AB-2A

   Plasmid

   #213627

   Purpose

   Bacterial expression of SUMO-tagged mouse syt7 C2AB-2A

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   syt7 C2AB-2A (Syt7 Mouse)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   I298, L361A

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET SUMO-FL-syt1 5W

   Plasmid

   #213629

   Purpose

   Bacterial expression of SUMO-tagged rat FL-syt1 5W

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt1 5W (Syt1 Rat)
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   M173W, F231W, F234W, C277A, V304W, Y364W, I367C

   Promoter

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFsynW SYT1deltaC2AB reporter

   Plasmid

   #197279

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-4T Syt1 C2AB(140) M173C

    Plasmid

    #134696

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    M173C, C277A

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-4T Syt1 C2AB(140) F234C

    Plasmid

    #134697

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F234C, C277A

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-4T Syt1 C2AB(140) I367C

    Plasmid

    #134699

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I367C, C277A

    Promoter

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

    Plasmid

    #167478

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(I539E)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

    Plasmid

    #167479

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(C450A)-ShadowG
    

    Use

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDD2027

    Plasmid

    #101370

    Purpose

    Name: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).

    Depositor

    Dirk Dittmer

    Article

    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

    Insert

    LANApi TATA box mutation in pCBR-K14 (pDD2005).
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    LANApi TATA box mutation in pCBR-K14 (pDD2005).

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid

    #158768

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179721

    Purpose

    Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

    Plasmid

    #179722

    Purpose

    Lentiviral expression of lysosome-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179723

    Purpose

    Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge