We narrowed to 13,035 results for: SHI

1. PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

   Plasmid

   #168290

   Purpose

   CRISPR-activator Suntag-P65-HSF1 (SPH)

   Depositor

   Hui Yang

   Article

   Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

   Insert

   dCas9, Suntag-P65-HSF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-ASAP3Kv-WPRE

   Plasmid

   #132330

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3KV
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-GFAP-NLS-CasRx-NLS-FLAG-U6-DR-SgRNA_Ptbp1-DR

   Plasmid

   #154001

   Purpose

   Vector expressed CasRx and sgRNA_Ptbp1 for AAV package

   Depositor

   Hui Yang

   Article

   Zhou et al Cell. 2020 Apr 30;181(3):590-603.e16. do

   Insert

   CasRx, Ptbp1 sgRNAs
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   GFAP, U6

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-N1-YAPS127A

   Plasmid

   #166458

   Purpose

   Expresses fusion of mEGFP and YAPS127A

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAPS127A (YAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. WT1-2A-eGFP PGK PuroR Donor Plasmid

   Plasmid

   #82333

   Purpose

   WT1 targeting donor

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Inserts

   WT1 5' Arm (WT1 Human)
   WT1 3' Arm (WT1 )
   

   Use

   CRISPR and TALEN

   Tags

   2A-eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-N1-YAP

   Plasmid

   #166457

   Purpose

   Expresses fusion of mEGFP and YAP

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAP (YAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

   Plasmid

   #197031

   Purpose

   Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized denOsCas12f1-VPR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-FBXL5-SKP1

   Plasmid

   #226620

   Purpose

   Co-expression of FBXL5-SKP1 complex in E.coli

   Depositor

   Ning Zheng

   Article

   Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

   Inserts

   FBXL5 (FBXL5 Human)
   SKP1 (SKP1 Human)
   

   Use

   Tags

   GB1

   Expression

   Bacterial

   Mutation

   Amino acids 1-198 was deleted, and 420-596 was re…

   Promoter

   T7

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. gTBEv4

   Plasmid

   #220620

   Purpose

   vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

   Insert

   nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

   Promoter

   hU6, EF1a

   Available since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-dCas12a-ABE8e-V106W

    Plasmid

    #193646

    Purpose

    Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TadA*8e-V106W

    Promoter

    CMV

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-enOsCas12f1-NLS_pA_AAV2 ITR

    Plasmid

    #197028

    Purpose

    AAV vector encoding a human codon-optimized enOsCas12f1 driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized enOsCas12f1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    D52R / T132R

    Promoter

    MHCK7, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLKO.1-YAP shRNA2

    Plasmid

    #166485

    Purpose

    Human YAP RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for human YAP (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX311-GFP-MEKDD

    Plasmid

    #194882

    Purpose

    Expression of dominant negative MEK1

    Depositor

    Sefi Rosenbluh

    Article

    Tuano et al Genome Biol. 2023 Mar 29;24(1):59. doi:

    Insert

    MEKDD (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S218D, S222D

    Promoter

    E1Fa

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Pet22b-YAP

    Plasmid

    #166443

    Purpose

    Bacterial expression of 6x His tagged YAP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. plentiCRISPRv2-PURO-LYSET-gRNA1

    Plasmid

    #210489

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA1 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. plentiCRISPRv2-PURO-LYSET-gRNA2

    Plasmid

    #210490

    Purpose

    CRISPR pooled KO of human LYSET

    Depositor

    Jan Carette

    Article

    Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

    Insert

    LYSET-gRNA2 (LYSET Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-C-EP300

    Plasmid

    #166454

    Purpose

    Expresses fusion of mCherry and EP300-C-IDR

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, C-EP300(1795-2414) (EP300 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLKO.1-Yap shRNA

    Plasmid

    #166486

    Purpose

    Mouse Yap RNAi

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    shRNA for mouse Yap (Yap1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-neo-YAP

    Plasmid

    #166468

    Purpose

    Plasmid coding for YAP and neomycin resistance in a lentiviral backbone.

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    YAP, Neo (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEGFP-N1-LATS2

    Plasmid

    #166474

    Purpose

    Expresses fusion of mEGFP and LATS2

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, LATS2 (LATS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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