Addgene

We narrowed to 10,569 results for: cat.1

Showing: 921 - 940 of 10569 results

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HaloTag-G protein gamma-1

Plasmid

#67038

Purpose

G protein gamma-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein gamma-1 (GNGT1 Bovine)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-Flag-RBM24-1~93

Plasmid

#208633

Purpose

for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

Depositor

Quanfu Ma

Insert

human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

only aa 1~93, other residues not included

Promoter

T7

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag-G protein beta-1

Plasmid

#67016

Purpose

G protein beta-1 tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-G protein beta-1 (GNB1 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-MKL1/2 shRNA

Plasmid

#27161

Purpose

Lentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

shRNA MKL1+2

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-1-H340D_enzymatic-domain

Plasmid

#183388

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

H340D

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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p8389 LentiCRISPRv2 Neo sgNT-1

Plasmid

#221649

Purpose

Expression of non-targeting control sgRNA

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

non-targeting sgNT-1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_eGFP

Plasmid

#82714

Purpose

Expresses Myosin VI HMM with a C-terminal eGFP.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

Myosin VI

Use

Tags

FLAG purification tag and eGFP

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-C (w324-1)

Plasmid

#17404

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-B (w323-1)

Plasmid

#17403

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pET28_canine-Caspase-1/4_enzymatic-domain

Plasmid

#183383

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b/GVcl 1-1066 /*TBM

Plasmid

#162781

Purpose

amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Gallus gallus vinculin (VCL Chicken)

Use

Tags

Expression

Bacterial

Mutation

changed Gly 454 to Val

Promoter

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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p8092 LentiCRISPR v2 sgNT-1

Plasmid

#163315

Purpose

Expresses Cas9 and a non-targeting control guide RNA

Depositor

Elizabeth White

Article

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

sgNT-1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-TRAF6-targeting sgRNA 1

Plasmid

#131345

Purpose

gRNA targeting mouse TRAF6

Depositor

Jonathan Kagan

Article

Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

Insert

gRNA targeting mouse TRAF6 (Traf6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex-1 Myosin VI HMM_SNAP

Plasmid

#82716

Purpose

Expresses Myosin VI HMM with a C-terminal SNAP tag.

Depositor

Sivaraj Sivaramakrishnan

Article

Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

Insert

Myosin VI

Use

Tags

6xHis Tag, FLAG purification tag, and SNAP tag

Expression

Bacterial and Insect

Mutation

Promoter

T7 Promoter

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGFP

Plasmid

#83085

Purpose

Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pACEBac1_Strep-Psc-BICD2(1-710)

Plasmid

#228831

Purpose

Insect cell expression of truncated BICD2 (aa 1-710)

Depositor

Andrew Carter

Article

Manigrasso et al bioRxiv 2024.04.11.589026

Insert

BICD2 (Bicd2 Mouse)

Use

Tags

Strep

Expression

Insect

Mutation

aa 1-710

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP -1

Plasmid

#194512

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP cLAP +1

Plasmid

#194509

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

cLAP(EGFP)-P2A-NGFR

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGTag NGFR EGFP nLAP -1

Plasmid

#194506

Purpose

CRISPR/Cas9-mediated generic protein tagging

Depositor

Frank Schnütgen

Article

Seibert et al MethodsX. 2022 Nov 18;9:101929. doi: 10.

Insert

NGFR-P2A-nLAP(EGFP)

Use

Bacterial cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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