We narrowed to 1,138 results for: fret

1. Vinculin-Clover-CC-Mad-mRuby2

   Plasmid

   #233239

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-Mad-mRuby2
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vh-Clover-CC-S1-mRuby2-Vt

   Plasmid

   #233230

   Purpose

   Vinculin FRET tension sensor with coiled-coil S1 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S1-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Vh-Clover-CC-S0-mRuby2-Vt

   Plasmid

   #233226

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S0-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Vh-Clover-CC-S4-mRuby2-Vt

   Plasmid

   #233228

   Purpose

   Vinculin FRET tension sensor with coiled-coil S4 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S4-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Vh-Clover-CC-S7-mRuby2-Vt

   Plasmid

   #233229

   Purpose

   Vinculin FRET tension sensor with coiled-coil S7 domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-S7-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Vh-Clover-CC-Mad-mRuby2-Vt

   Plasmid

   #233232

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vh-Clover-CC-Mad-mRuby2-Vt
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Vinculin-Clover-CC-S0-mRuby2

   Plasmid

   #233233

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S0-mRuby2
   

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Tags

   CFP and YFP

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPBbsr2-TSen

   Plasmid

   #209916

   Purpose

   A FRET biosensor for ADAM17 activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TSen
   

   Tags

   ECFP and YPet

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p35S::SP-mCherry-GFP-HDEL

    Plasmid

    #159097

    Purpose

    Positive control for FRET in plant ER / nuclear envelope.

    Depositor

    Joe McKenna

    Article

    McKenna et al Front Plant Sci. 2021 Feb 17;12:645218.

    Insert

    SP-mCherry-GFP-HDEL
    

    Tags

    mCherry, GFP

    Expression

    Plant

    Promoter

    35S

    Available Since

    Oct. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDx_FusionRed-SYFP2

    Plasmid

    #189760

    Purpose

    Dual expression vector for bacteria and mammalian cells producing FusionRed-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    FusionRed-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDx_mScarlet-SYFP2

    Plasmid

    #189762

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCherry-FKBP-E2

    Plasmid

    #175243

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

    Plasmid

    #138197

    Purpose

    FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid

    #138198

    Purpose

    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and cpV[E172]

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

    Plasmid

    #138199

    Purpose

    FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains arginine-to-alanine substitution at resi…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

    Plasmid

    #138200

    Purpose

    FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

    Depositor

    Jin Zhang

    Article

    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

    Insert

    Cerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
    

    Tags

    Cerulean and Venus

    Expression

    Mammalian

    Mutation

    Contains leucine-to-tryptophan substitution at re…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-TORCAR

    Plasmid

    #64927

    Purpose

    FRET-based biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR (EIF4EBP1 Synthetic, Human)
    

    Tags

    Cerulean and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Lyn-ICUE3

    Plasmid

    #61623

    Purpose

    Plasma membrane-targeted FRET biosensor for monitoring cAMP

    Depositor

    Jin Zhang

    Article

    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

    Insert

    Lyn-ICUE3 (RAPGEF3 Human)
    

    Tags

    ECFP, N-terminal targeting sequence from Lyn kina…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge