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Showing: 921 - 940 of 1122 results

  1. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

    Plasmid
    #138198
    Purpose
    FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.
    Depositor
    Jin Zhang
    Article
    Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi
    Insert
    Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Mouse, Synthetic)
    Use
    Tags
    Cerulean and cpV[E172]
    Expression
    Mammalian
    Mutation
    Contains arginine-to-alanine substitution at resi…
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138198-plasmid-map-sequence-id-307497

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  4. pcDNA3-PKAsub-YPet-Kras

    Plasmid
    #138206
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKAsub-YPet-Kras
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Availability
    Academic Institutions and Nonprofits only
    138206-plasmid-map-sequence-id-307409

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  11. pcDNA3-endo-EKAR4

    Plasmid
    #205513
    Purpose
    Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-EKAR4
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205513-plasmid-map-sequence-id-409396

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  16. pSOMA-NES

    Plasmid
    #118939
    Purpose
    Used to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118939-plasmid-map-sequence-id-232111

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  19. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Article
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172473-plasmid-map-sequence-id-342817

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  20. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

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Showing: 921 - 940 of 1122 results