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pFLAG W96A-GLTP

Plasmid

#170741

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

GLTP (GLTP Human)

Tags

FLAG

Expression

Mammalian

Mutation

W96A

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-nsp5

Plasmid

#168868

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-nsp5 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-2-ORF8

Plasmid

#168884

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-2-ORF8 (ORF8 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr-D435A/E437A

Plasmid

#40829

Purpose

Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

Depositor

Phillip Zamore

Article

Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

Insert

Nbr D435A/E437A (Nbr Fly)

Tags

3xFLAG and 6xMyc

Expression

Insect

Mutation

D435A/E437A, part of the DEDD motif; mutations p…

Promoter

Actin5C

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pEntry_HCoV-229E-nsp12

Plasmid

#168969

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp12 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFLAG CPTP

Plasmid

#170742

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Gao et al PLoS One. 2011;6(5):e19990. doi: 10.1371

Insert

CPTP (CPTP Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-hCRACR2A-isoform-c

Plasmid

#79591

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

Insert

CRACR2A-isoform-c (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
GFP-SP110A

Plasmid

#65392

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

SP110A

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
lentiCRISPR.sgKras.9

Plasmid

#91894

Purpose

sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

Depositor

Wen Xue

Article

Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Insert

Kras sgRNA

Use

Lentiviral

Promoter

U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-NL63 E_stop

Plasmid

#168684

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-NL63 E_nostop

Plasmid

#168666

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEntry_HCoV-NL63-Envelope

Plasmid

#168924

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Envelope (HCNV63gp4 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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p7612 MSCV-P C-FlagHA 16E7 E10K

Plasmid

#163303

Purpose

Expresses HPV16 E7 E10K

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 E10K

Use

Retroviral

Tags

FlagHA

Mutation

E10K

Promoter

MSCV LTR

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp8

Plasmid

#168957

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp8 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp8

Plasmid

#168897

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp8

Plasmid

#168850

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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