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Control-cx43.4-48H-pGTag

Plasmid

#117818

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-STK11

Plasmid

#116794

Purpose

Lentiviral expression of STK11

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

STK11 (STK11 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CD

Plasmid

#116773

Purpose

Lentiviral expression of PIK3CD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CD (PIK3CD Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-DDR2

Plasmid

#116729

Purpose

Lentiviral expression of DDR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DDR2 (DDR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-B2M

Plasmid

#116715

Purpose

Lentiviral expression of B2M

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

B2M (B2M Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLXI_TRC401 SMARCB1 var 2

Plasmid

#111182

Purpose

Inducible vector (untagged) with SMARCB1 variant 2

Depositor

William Hahn

Article

Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

Insert

BAF47 (SMARCB1 Human)

Use

Lentiviral

Promoter

TRE

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLIX405-KANK1

Plasmid

#121984

Purpose

tetracycline-inducible expression of C-terminal GFP tagged KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Tags

Emerald GFP

Promoter

Tight TRE promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCWXPG-UBI-MafA

Plasmid

#114302

Purpose

Constitutive expression of the MafA trancription factor

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

MafA (MAFA Human)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-P278A

Plasmid

#118015

Purpose

Constitutive expression of mutant human Tumor Protein p53 (TP53)

Depositor

William Hahn, David Root

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

p.Pro278Ala

Promoter

EF1alpha

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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Pinducer20-BSD-scFv-sfGFP-DNMT3A1

Plasmid

#115780

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

scFv-sfGFP-DNMT3A1 (DNMT3A Human)

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pg-EGFP-HIF-2alpha

Plasmid

#87207

Purpose

N-terminally EGFP-tagged human HIF-2a/EPAS

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-2a (EPAS1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q868E

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP-SHLD3

Plasmid

#114126

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-tagged SHLD3

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD3 (SHLD3 Human)

Use

Lentiviral; Doxycycline inducible

Tags

eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-eGFP-FHA-SHLD2

Plasmid

#114121

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MAC_C_AOX

Plasmid

#111661

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AOX

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEXP(V5-APEX2-NES)

Plasmid

#107596

Purpose

Expresses V5-APEX2-NES in mammalian cells

Depositor

Markus Hafner

Article

Benhalevy et al Nat Methods. 2018 Dec;15(12):1074-1082.

Insert

APEX2-NES

Use

Frt site to insert into hek293 t-rex genome

Tags

NES and V5

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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MX2_pCSdest

Plasmid

#53942

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MX2 (MX2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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DSCAM_pCSdest

Plasmid

#53906

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DSCAM (DSCAM Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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USP25_pCSdest

Plasmid

#53817

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

USP25 (USP25 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX307 hDVL1

Plasmid

#102863

Purpose

Expresses human DVL1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

human Dishevelled 1 (DVL1) (DVL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 2 to glycine. Likely due to poly…

Promoter

EF1A

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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