We narrowed to 14,476 results for: NTS

1. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

   Plasmid

   #169466

   Purpose

   Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-cDAXX-0SB

   Plasmid

   #169730

   Purpose

   Bacterial expression of N-terminally 6His tagged cDAXX-0SB

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   cDAXX-0SB (DAXX Human)
   

   Tags

   6 His

   Expression

   Bacterial

   Mutation

   Comprises residues 495-741 and has the major SB m…

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p35S_EVDm1

   Plasmid

   #167121

   Purpose

   Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE (EVD) (AT5G17125 Mustard Weed)
   

   Expression

   Plant

   Mutation

   mutated four nucleotides at the snoRNA U1 binding…

   Promoter

   CaMV 35S

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

   Plasmid

   #166814

   Purpose

   Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1322R

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S_GFP-EVDinter-GUS

   Plasmid

   #167123

   Purpose

   Plant binary expression vector containing the intron and proximal polyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.

   Depositor

   Arturo Marí-Ordóñez, Olivier Voinnet

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
   

   Tags

   GUS and mGFP5

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E

   Plasmid

   #166811

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contacts

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   LL1193EE, P1214R, K1277E

   Promoter

   CMV

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVD1 M3_3-pTRNA-scf 2.1 (GB2075)

   Plasmid

   #160560

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M3_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M3_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

   Plasmid

   #160558

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G

    Plasmid

    #166812

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    H1276G

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E

    Plasmid

    #166813

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K1277E

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

    Plasmid

    #160559

    Purpose

    tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    M2_3-pTRNA-scf 2.1
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Feb. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUPD2 sgRNA sCRNA 2.0 (GB2461)

    Plasmid

    #160593

    Purpose

    Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA sCRNA 2.0
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

    Plasmid

    #160625

    Purpose

    GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26-1gRNA-DFR 2.1
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLIK 3xFLAG-MAVS-Ala271 hygro

    Plasmid

    #158637

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Mutation

    Q271A

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

    Plasmid

    #158638

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Mutation

    E93Q/Q271A

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3xFLAG-MAVS-Gln93,Ala271

    Plasmid

    #158632

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Mutation

    E93Q/Q271A

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TT 3xFLAG-MAVS-Ala148,Ala271

    Plasmid

    #158633

    Purpose

    Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Gateway entry vector

    Tags

    3XFLAG

    Mutation

    Q148A/Q271A

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

    Plasmid

    #158639

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Mutation

    Q148A/Q271A

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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