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Hv_HPT:pCSRM2C:Gus_introns

Plasmid

#215191

Purpose

Evaluating guard cell specific promotors in Barley

Depositor

Penny Hundleby

Article

Nguyen et al Plant Direct. 2025 Feb 12;9(2):e70045. d

Insert

Scream2

Promoter

BdSCRM2

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pYO524

Plasmid

#235744

Purpose

Protein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome

Depositor

Roger Williams

Article

Baretic et al Nat Commun. 2016 Apr 13;7:11016. doi: 10

Insert

KmLST8

Expression

Yeast

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_GFP

Plasmid

#235611

Purpose

Encodes GFP. For use in transfections as a negative control without epitope tags.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pVP226

Plasmid

#222056

Purpose

GreenGate AE bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP227

Plasmid

#222057

Purpose

GreenGate AF bridge module containing filller sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP228

Plasmid

#222058

Purpose

GreenGate BD bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP229

Plasmid

#222059

Purpose

GreenGate BE bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP230

Plasmid

#222060

Purpose

GreenGate CD bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP231

Plasmid

#222061

Purpose

GreenGate CE bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP295

Plasmid

#222064

Purpose

GreenGate AB base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP296

Plasmid

#222065

Purpose

GreenGate BC base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP297

Plasmid

#222066

Purpose

GreenGate CD base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP298

Plasmid

#222067

Purpose

GreenGate DE base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP299

Plasmid

#222068

Purpose

GreenGate EF base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP300

Plasmid

#222069

Purpose

GreenGate FG base entry vector with an Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

Domesticated of Esp3I sites

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP096

Plasmid

#222043

Purpose

pGGPK-AG2 where the ccdb/CmR has been swappe for mRFP1e; Esp3I domesticated backbone.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

mRFP1e

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP333

Plasmid

#222044

Purpose

GreenGate AB bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pVP334

Plasmid

#222045

Purpose

GreenGate FG bridge module containing filler sequence.

Depositor

Wayne Parrott

Article

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Insert

Filler

Use

Synthetic Biology; Greengate compatible cloning v…

Available Since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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