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pBR25 CFAP36 [L438E, K442E]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238227

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [L438E, K442E], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Publication

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [L438E, K442E]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 CFAP36 [A493E, K495A]-3C-mStayGold-2Strep crBSDr (C. reinhardtii)

Plasmid

#238228

Purpose

CRISPR donor plasmid for C-terminal tagging and knock-in of CFAP36 [A493E, K495A], and insertion of Blasticidin resistance (C. reinhardtii)

Depositor

Alan Brown

Publication

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

CFAP36 [A493E, K495A]

Use

CRISPR; Donor

Tags

-3C-mStayGold-2Strep

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBR25 IFT70 [A331R, A335R] crSpecR (C. reinhardtii)

Plasmid

#238230

Purpose

CRISPR donor plasmid for C-terminal knock-in of IFT70 [A331R, A335R] and insertion of Spectinomycin resistance (C. reinhardtii)

Depositor

Alan Brown

Publication

Lange et al Cell. 2025 Nov 13;188(23):6492-6508.e18.

Insert

IFT70 [A331R, A335R]

Use

CRISPR; Donor

Tags

None

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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REKAR76-TA

Plasmid

#225705

Purpose

T/A Mutant of REKAR76, Non-phosphorylatable version.

Depositor

John Albeck

Publication

DeCuzzi et al J Biol Eng. 2025 Nov 13;19(1):102. doi:

Insert

REKAR76-TA

Tags

miRFP670nano3 and miRFP720

Expression

Bacterial and Mammalian

Mutation

Threonine 498 mutated to Alanine

Promoter

hEF-1a

Available since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab813-bGlobin_3xF-BRCA1(full-length)-Y1845D

Plasmid

#249024

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845D mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845D

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab814-bGlobin_3xF-BRCA1(full-length)-M1775R

Plasmid

#249025

Purpose

This plasmid expresses the full length BRCA1 sequence (M1775R mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

M1775R

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab816-bGlobin_3xF-BRCA1(full-length)-G1788V

Plasmid

#249026

Purpose

This plasmid expresses the full length BRCA1 sequence (G1788V mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

G1788V

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab818-bGlobin_3xF-BRCA1(full-length)-A1708E

Plasmid

#249027

Purpose

This plasmid expresses the full length BRCA1 sequence (A1708E mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

A1708E

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab819-bGlobin_3xF-BRCA1(full-length)-Y1845F

Plasmid

#249028

Purpose

This plasmid expresses the full length BRCA1 sequence (Y1845F mutation) with an N-terminal 3xFLAG tag

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Tags

3x-FLAG-V5

Expression

Mammalian

Mutation

Y1845F

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab676-HBEGF-2a-mWasabi-WPRE

Plasmid

#249029

Purpose

This plasmid expresses the mWasabi flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mWasabi

Use

CRISPR

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab639-HBEGF-2a-mRuby3-WPRE

Plasmid

#249030

Purpose

This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mRuby3

Use

CRISPR

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab672-HBEGF-2a-mTagBFP2-WPRE

Plasmid

#249031

Purpose

This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mTagBFP2

Use

CRISPR

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab821-pcDNA3.1-HALO-3xFLAG

Plasmid

#249007

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

HALO

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab966-pUC19-HAL-BRCA1-BSD-P2A-2xHA-FKBP12-F36V-HAR-BRCA1

Plasmid

#249004

Purpose

This plasmid expresses the degron tag knock-in sequence at the N-terminus of the endogenous BRCA1 locus

Depositor

Georgios Karras

Publication

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

FKBP12 (FKBP1A Human)

Use

CRISPR

Tags

2xHA

Available since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1370_pTet-ECOR31_Hachiman

Plasmid

#223364

Purpose

Expression of ECOR31 Hachiman under the pTet promoter

Depositor

Jennifer Doudna

Publication

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman

Expression

Bacterial

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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MFN2-Halo donor plasmid

Plasmid

#239866

Purpose

endogenously tags MFN2 with Halo tag

Depositor

Derek Narendra

Publication

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

MFN2 (MFN2 Human)

Use

Donor plasmid

Tags

Halo Tag

Available since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Plasmid

#238894

Purpose

EMcapsulin class with a eUnaG fusion and an anti-mCherry intrabody (LaM-4) fusion preceded by a TGA stop codon and the rt20s readthrough motif.

Depositor

Gil Westmeyer

Publication

Berezin et al Nat Protoc. 2025 Nov 19. doi: 10.1038/s4

Insert

1M-Qt-eUnaG-TGA-rt20s-anti-mCherry(LaM-4)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EML6442_p35S-mCherry2-SV40

Plasmid

#234351

Purpose

Expressed mCherry2 florescent proteins in plant cells for tracing transformation events

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

SpeR

Expression

Plant

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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EML8359_pA3A-CBECas9-ALSpro197

Plasmid

#234353

Purpose

Base editor targets to ALS Pro197 locus

Depositor

Erh-Min Lai

Publication

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Insert

ALS

Use

CRISPR

Expression

Plant

Available since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHS-MRscRNA

Plasmid

#240214

Purpose

Mammalian vector for transient expression of theophylline-responsive MR-scRNA with mCherry transfection marker

Depositor

Wilfred Chen

Publication

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TET3G promoter

Expression

Mammalian

Promoter

U6

Available since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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