Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 27,787 results for: STI

Showing: 9521 - 9540 of 27787 results

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-C

Plasmid

#128309

Purpose

Express hNABP2 C-terminus(122–211 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 C-terminal domain (122–211 aa) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

C-terminal domain (122–211 aa)

Promoter

T7

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly-4xUAS

Plasmid

#125149

Purpose

Vector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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JL104: pMVP/Neo-DEST

Plasmid

#121857

Purpose

pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_Trf2

Plasmid

#125159

Purpose

Gateway entry clone

Depositor

Julius Brennecke, Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

Trf2 (Trf2 Fly)

Use

Gateway entry vector

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(F317D, I325A)

Plasmid

#122275

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325A)

Plasmid

#122274

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D, I325A)

Plasmid

#122273

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid; Isoleucine 32…

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(I325A)

Plasmid

#122272

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to alanine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317Q)

Plasmid

#122271

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glutamine

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317D)

Plasmid

#121391

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317G)

Plasmid

#122268

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to glycine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(F317K)

Plasmid

#122270

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Phenylalanine 317 to lysine

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆335-417)

Plasmid

#121525

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

deleted amino acids 335-417*

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(L105D)

Plasmid

#121395

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGBDU-Atg17(I325D)

Plasmid

#121397

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(L331D)

Plasmid

#121393

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 331 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(L105D)

Plasmid

#121388

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Leucine 105 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(I325D)

Plasmid

#121392

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 325 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(I310D)

Plasmid

#121389

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Mutation

Isoleucine 310 to Aspartic acid

Promoter

ADH1 promoter

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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