We narrowed to 17,211 results for: EMB

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mCherry-SH3(5R)

Plasmid

#112154

Purpose

Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3-5R (NCK1 Human)

Expression

Mammalian

Mutation

only the second SH3 domain is expressed in this c…

Promoter

CMV

Available Since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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5xNF-kb_RE::RedF

Plasmid

#124530

Purpose

NFKB RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

NF-κβ RedF reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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Lyn FAK biosensor

Plasmid

#78299

Purpose

FRET biosensor. Visualization of FAK activity at lipid rafts in live cells

Depositor

Yingxiao Wang

Article

Seong et al Nat Commun. 2011 Jul 26;2:406. doi: 10.1

Insert

Lyn Fak biosensor

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-beta-catenin-S33Y

Plasmid

#13371

Purpose

Retroviral expression of mouse beta-catenin S33Y mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

catenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Serine 33 to Tyrosine and Leucine 388 to Proline

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pHBS838 TDP43 RRM-GFP

Plasmid

#98249

Purpose

TDP43 RRM-GFP reporter construct

Depositor

Rajat Rohatgi

Article

Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

Insert

TARDBP (TARDBP Human)

Tags

Internal GFP to replace RRM domains

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PIP5K1C (PIP5K1C Human)

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CETN2

Plasmid

#227291

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CETN2 (CETN2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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SP-GFP-CD274-NU

Plasmid

#121139

Purpose

Mammalian expression of GFP tagged CD274

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Inserts

CD274 (CD274 Human)
CD274 (CD274 Human)

Expression

Mammalian

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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T-STOP sgRNA-1

Plasmid

#83346

Purpose

The sgRNA at the stop codon of human Brachyury(T) locus

Depositor

James Thomson

Article

Chu et al Genome Biol. 2016 Aug 17;17(1):173. doi:

Insert

T-STOP sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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IL2Rα-EGFP

Plasmid

#86055

Purpose

A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Interleukin-2 receptor subunit alpha (IL2RA Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKH115_GALNT2-keima

Plasmid

#214969

Purpose

Golgiphagy flux reporter GALNT2-keima

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GALNT2-keima (GALNT2 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

Plasmid

#187356

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo.ARRDC1-mCherry

Plasmid

#38319

Depositor

Quan Lu

Article

Nabhan et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

ARRDC1 (ARRDC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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SDHC-pHLuorin-sems

Plasmid

#107767

Purpose

labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

Depositor

Karin Busch

Article

Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

Insert

Subunit C of SDH fused to pHluorin (SDHC Human)

Tags

pHluorin

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_HA-BirA-ER

Plasmid

#113897

Purpose

Mammalian (inducible) expression of HA-tagged ER-resident E. coli biotin ligase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

E. coli biotin ligase

Use

Lentiviral

Tags

HA

Promoter

CMV-MIE-TetO2

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Mucolipin1-pEGFP C3

Plasmid

#62960

Purpose

full length human Mucolipin-1 cloned into pEGFP C3

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Mucolipin-1 has 1 silent mutation compared to the…

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-Abl-His6-FLAG

Plasmid

#52684

Purpose

expression of Abl in mammalian cells

Depositor

Benjamin Turk

Article

Deng et al Chembiochem. 2012 Nov 26;13(17):2510-2.

Insert

ABL1 (Abl1 Mouse)

Tags

FLAG and His6

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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EGFP-CLC

Plasmid

#193559

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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