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pDGE657

Plasmid

#153255

Purpose

M2E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Publication

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-R2-PBR-L4

Plasmid

#141017

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR2-L3-PBL-R1

Plasmid

#141016

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-P5P4

Plasmid

#141064

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMM1162

Plasmid

#112535

Purpose

pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Publication

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pZS1katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78217

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pProm9_BCD1-GFP

Plasmid

#80655

Purpose

Promoter9 with BCD1-gfp

Depositor

Nathan Hillson

Publication

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCR4-MBII52-probe

Plasmid

#67647

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Publication

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII52 snoRNA (Snord115 Mouse)

Use

For in vitro transcription

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pET:GACG:GFP

Plasmid

#60735

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GACG' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pISH-V1rb1

Plasmid

#16010

Depositor

Peter Mombaerts

Publication

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

V1rb1 (Vmn1r50 Mouse)

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Py-MDR2-NBD

Plasmid

#25590

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Py-MDR2-NBD

Tags

His

Expression

Bacterial

Available since

Aug. 25, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBAD-luxKate

Plasmid

#229946

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)

Depositor

Andy Yeh

Publication

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxKate

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Expression

Yeast

Promoter

SSA1 S.c.

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Publication

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMVS1111A:PhmtB-bgaB

Plasmid

#205998

Purpose

Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

Depositor

Bastian Molitor

Publication

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Insert

thermostable beta-galactosidase

Promoter

PhmtB

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-blast

Plasmid

#208033

Purpose

Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-puro

Plasmid

#208031

Purpose

Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Publication

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Expression

Worm

Mutation

None

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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