We narrowed to 7,351 results for: Lif;

1. pLgw NKX2-5-V5-EcoDam

   Plasmid

   #98597

   Purpose

   Mammalian DamID lentiviral vector for NKX2-5 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR8/GW/TOPO-ELK1Y158A

   Plasmid

   #98628

   Purpose

   Gateway entry TOPO TA cloning vector ELK1 Y158A

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 Y158A (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Y158A

   Promoter

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8/GW/TOPO-ETS1

   Plasmid

   #98621

   Purpose

   Gateway entry TOPO TA cloning vector for ETS1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ETS1 (Ets1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS

   Plasmid

   #60847

   Purpose

   Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

   Depositor

   Jamie Cate

   Article

   Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

   Inserts

   S. pyogenese Cas9
   RNA pol III promoter (tRNA-Tyr)
   hepatitis delta virus ribozyme, genomic
   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS/His8 and TTT 3' extension prior to sgRNA

   Expression

   Bacterial and Yeast

   Mutation

   L4 is UUCG tetraloop and guide targets LYP1 (CATA…

   Promoter

   Available since

   Jan. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLgw EcoDam-V5-ELK1

   Plasmid

   #98604

   Purpose

   Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1.1-mCherry

   Plasmid

   #162772

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   red fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1.2-GS-eGFP

   Plasmid

   #162771

   Purpose

   Fluorescent reporter for CHO expression studies

   Depositor

   Ivan Vorobiev

   Article

   Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

   Insert

   enhanced green fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   consensus Kozak sequence (GCCGCCATGG) added befor…

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. SITS-MmCav1-APEX2

   Plasmid

   #170526

   Purpose

   Vector expression of MmCav1-APEX2 in Leishmania cell free system. Parton lab clone IYW

   Depositor

   Rob Parton

   Article

   Rae et al Elife. 2021 Apr 27;10. pii: 64630. doi:

   Insert

   Caveolin1 (Cav1 Mouse)
   

   Use

   Leishmania cell free expression clone

   Tags

   APEX2 and Lifeact

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLgw EcoDam-V5-NKX2-5Y191C

   Plasmid

   #98598

   Purpose

   Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 (Nkx2-5 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Y191C point mutant

   Promoter

   Heat Shock Minimal Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLgw NKX2-5Y191C-V5-EcoDam

    Plasmid

    #98599

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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