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Tol2-lyzC-microRNA 24-1-Dendra2

Plasmid

#97106

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 24 1 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 31-Dendra2

Plasmid

#97107

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 31 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 96--dendra2

Plasmid

#97108

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 96 dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 99-1-Dendra2

Plasmid

#97109

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 99 1 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRSM41

Plasmid

#113940

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor REX-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

REX-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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CC1-Pos6

Plasmid

#101656

Purpose

CC1-Pos6 is a modified version of CC1-M240 that has 4 arginines in its linker. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

CC1-Pos6

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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CC1-M240

Plasmid

#101657

Purpose

CC1 with shorter linker length and the 'GDP' fusion site. (* super ecliptic pHluorin A227D)

Depositor

Bradley Baker

Article

Lee et al Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.

Insert

CC1-M240

Expression

Mammalian

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AN+

Plasmid

#78240

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS-

Plasmid

#78239

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 AS+

Plasmid

#78236

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDHJS3 BS+

Plasmid

#78252

Purpose

One of four plasmids needed to create the double Holliday junction substrate (DHJS).

Depositor

Tao-shih Hsieh

Article

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "B"
loxP2

Use

Phagemid

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCVD403

Plasmid

#63740

Purpose

Contains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184

Depositor

James Kaper

Article

Gicquelais et al J Clin Microbiol. 1990 Nov;28(11):2485-9

Insert

LT gene probe

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pGLx2-set1-H1017K

Plasmid

#53258

Purpose

GAL driven LexA-tagged SET1-catalytically dead (H1017K)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

H1017K mutation - catalytically dead

Promoter

GAL1/10

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B11-Leum

Plasmid

#44319

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B11-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B11-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B1-Leum

Plasmid

#44303

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B1-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B1-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B2-Leum

Plasmid

#44310

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B2-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B2-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B3-Leum

Plasmid

#44311

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B3-Leum promoter

Use

Synthetic Biology

Tags

N/A

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B3-Leum

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-PM-BAF180-SH3

Plasmid

#41933

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM-BAF180-SH1

Plasmid

#41931

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B9-Leum

Plasmid

#44317

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B9-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B9-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B7-Leum

Plasmid

#44315

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B7-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B7-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B15-Leum

Plasmid

#44323

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B15-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B15-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B14-Leum

Plasmid

#44322

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B14-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B14-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B13-Leum

Plasmid

#44321

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B13-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B13-Leum

Available Since

April 17, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-rB50

Plasmid

#18957

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican N-terminus (Bcan Rat)

Expression

Mammalian

Mutation

Brevican N-terminus, aminoacids 1-395

Available Since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-H2B-PAmCherry

Plasmid

#133419

Purpose

Fluorescent reporter for calcium imaging and photoactivation

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-H2B-PAmCherry (H2BC11 Human)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Mutation

N28D in mCherry- please see depositor comments be…

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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