We narrowed to 5,032 results for: plasmid dna

1. pEGFP-C1-FLAG

   Plasmid

   #46956

   Purpose

   Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPAGFP-N1

   Plasmid

   #11909

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   pPAGFP-N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPAGFP-C1

   Plasmid

   #11910

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

   Has Service

   Cloning Grade DNA

   Insert

   PAGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   These plasmids are essentially the same as the Cl…

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPE4max-Blasticidin

   Plasmid

   #194905

   Purpose

   Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

   Depositor

   George Church

   Article

   Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

   Insert

   BSD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

   Plasmid

   #200637

   Purpose

   Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   Vif
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T27stop

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVITRO1-SS-338

   Plasmid

   #68795

   Purpose

   Single construct Sensor pair 1, ZF9 Op x6 -- GFP, with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   C-Terminal Sensor 1 V1
   mCherry
   ZF9 Op x6
   EGFP
   N-Terminal Sensor 1 V2
   

   Use

   Tags

   IRES, NLS, flag, and nls

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 53BP1 N-terminal sgRNA

   Plasmid

   #207091

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGGGAGCAGATGGACCCTAC sgRNA to target the 53BP1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGGGAGCAGATGGACCCTAC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BSP605

   Plasmid

   #122250

   Purpose

   expression vector for mCerulean under EFT3 promoter

   Depositor

   Alexander Mendenhall

   Article

   Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

   Insert

   mCerulean
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   EFT3

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BSP610

    Plasmid

    #122255

    Purpose

    expression vector for mtagBFP2 under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagBFP2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV_TALE-TF(VP64)-BB_V3

    Plasmid

    #42581

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    TALE-TF(VP64)-BB
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG hybrid

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ZFD_TRAC-NC-Right-G1397-C

    Plasmid

    #180773

    Purpose

    Expresses ZFD_TRAC-NC-Right-G1397-C(DddAtox half) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

    Insert

    ZFD for TRAC mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ZFD_TRAC-NC-Left-G1397-N

    Plasmid

    #180772

    Purpose

    Expresses ZFD_TRAC-NC-Left-G1397-N(DddAtox half) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

    Insert

    ZFD for TRAC mutation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BSP611

    Plasmid

    #122256

    Purpose

    expression vector for mtagRFP-T under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mtagRFP-T
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPSW1KN0034

    Plasmid

    #196178

    Purpose

    MoClo Level 1 luciferase reporter: CaMV35S::TMV::LucF::flag::35sT

    Depositor

    Nicola Patron

    Article

    Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

    Insert

    CaMV35S::TMV::LucF::flag::35sT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BSP606

    Plasmid

    #122251

    Purpose

    expression vector for mECitrine under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mECitrine
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAD01 S. cerevisiae telomerase RNA KBS

    Plasmid

    #61890

    Purpose

    Plasmid for run-off in vitro transcription of Saccharomyces cerevisiae KBS RNA

    Depositor

    Thomas Cech

    Article

    Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

    Insert

    Telomerase RNA hairpin
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGL2-Full

    Plasmid

    #16012

    Depositor

    Wafik El-Deiry

    Article

    Takimoto et al Oncogene. 2000 Mar 30. 19(14):1735-43.

    Insert

    KILLER/DR5 (TNFRSF10B Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. ATM N-terminal sgRNA

    Plasmid

    #207089

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATCATTAAGTACTAGACTCA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATCATTAAGTACTAGACTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CP2-TCC

    Plasmid

    #179679

    Purpose

    Complementary plasmid

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [sd2] T7 RNAP-TCC linker-degron
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRGM1

    Plasmid

    #48789

    Purpose

    HA-I-SceI expression plasmid, episomal

    Depositor

    Michael Glickman

    Article

    Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

    Insert

    I-SceI
    

    Use

    Tags

    HA tag

    Expression

    Bacterial

    Mutation

    Promoter

    MOP

    Available since

    Jan. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. psgRNA-ccdB

    Plasmid

    #99915

    Purpose

    Basic cloning vector for the expression of imaging-based optimization sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BSP608

    Plasmid

    #122253

    Purpose

    expression vector for mNeptune under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mNeptune
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSHS293

    Plasmid

    #87348

    Purpose

    D435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/D435C/C574S/E945C

    Promoter

    T7

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJC175e-DddI

    Plasmid

    #179693

    Purpose

    Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSHS248

    Plasmid

    #87354

    Purpose

    S867C/N1054C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-2 FRET construct

    Depositor

    Jennifer Doudna

    Article

    Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

    Insert

    Cas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    C80S/C574S/S867C/N1054C

    Promoter

    T7

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV_TALEN-FokI(WT)-BB_V3

    Plasmid

    #42588

    Depositor

    Feng Zhang

    Article

    Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

    Insert

    TALEN-FokI(WT)-BB
    

    Use

    AAV; Adeno-associated virus ()

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG hybrid

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. BSP607

    Plasmid

    #122252

    Purpose

    expression vector for mKO2 under EFT3 promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BSP602

    Plasmid

    #122249

    Purpose

    expression vector for mScarlet under EFT3 Promoter

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Worm

    Mutation

    Promoter

    EFT3

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDHJS3 AN-

    Plasmid

    #78241

    Purpose

    One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

    Inserts

    loxP
    sequence "A" with NheI site
    loxP2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDHJS3 AS-

    Plasmid

    #78237

    Purpose

    One of four plasmids needed to create the double Holliday junction substrate (DHJS).

    Depositor

    Tao-shih Hsieh

    Article

    Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

    Inserts

    loxP
    sequence "B"
    loxP2
    

    Use

    Phagemid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFP125

    Plasmid

    #21674

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been completely dele…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

33. RNF168 C-terminal sgRNA

    Plasmid

    #207100

    Purpose

    pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GAGATGCACAAAGTAAGGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RIF C-terminal sgRNA

    Plasmid

    #207096

    Purpose

    pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AATACTAAATAGAATTTTCA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. REV7 C-terminal sgRNA

    Plasmid

    #207095

    Purpose

    pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GCTCATAAAGGCAGCTGAGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SHLD3 N-terminal sgRNA

    Plasmid

    #207094

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTACTGCAGAATGACTACAG sgRNA to target the SHLD3 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTACTGCAGAATGACTACAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NBS1 C-terminal sgRNA

    Plasmid

    #207092

    Purpose

    pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TAAAAAGGAGAAGATAACTG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. RNF169 C-terminal sgRNA

    Plasmid

    #207099

    Purpose

    pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ACACTTCATTAGGTGCTACT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SHLD1 N-terminal sgRNA

    Plasmid

    #207101

    Purpose

    pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    ATGGCAGGACTATGGCAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ATM C-terminal sgRNA

    Plasmid

    #207097

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTCTAAAGGCTGAATGAAA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge