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3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
M50-IRES-GFP-IRES-tauLacZ LNL TV

Plasmid

#15646

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M50-IRES-GFP-IRES-tauLacZ LNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pRB306

Plasmid

#8666

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRB2070

Plasmid

#8671

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 with LYS2 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut I

Plasmid

#11322

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation I (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
OE2-M71

Plasmid

#15630

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

OE2-M71-IRES-tauGFP ACNF TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pJC-lecg

Plasmid

#239180

Purpose

riboprobes (SP6 or T3) and dsRNA (T7)

Depositor

Phillip Newmark

Article

Khan et al Cell Rep. 2022 Mar 15;38(11):110525. doi

Insert

lecg

Use

T/a cloning vector for dsrna generation and the g…

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAB228i16

Plasmid

#209738

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i24

Plasmid

#209739

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228r3

Plasmid

#209741

Purpose

ColA BBa_B0014 tetAp SD8 Lum1PylRS-[PylHRS*] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

Mlum1RS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p

Plasmid

#209742

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p4

Plasmid

#209743

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[AF] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB174

Plasmid

#101673

Purpose

Opto-T7RNAP*(179)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(179)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB144

Plasmid

#101665

Purpose

Opto-T7RNAP*(69)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAB153

Plasmid

#101664

Purpose

Opto-T7RNAP*(600)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(600)

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSKA322

Plasmid

#101676

Purpose

empty backbone with chloramphenicol resistance

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS621

Plasmid

#188893

Purpose

ttTi5605 MosSci targeting vector with floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Mossci targeting vector

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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