Addgene

We narrowed to 10,399 results for: CHL

Showing: 941 - 960 of 10399 results

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pC031 - RcrCas13a from Rhodobacter capsulatus R121

Plasmid

#91921

Purpose

Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcrCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

Plasmid

#91917

Purpose

Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LweCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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FUP95PDZGW (C5)

Plasmid

#74021

Purpose

lentiviral expression of Psd95-EGFP fusion

Depositor

Oliver Schluter, Robert Malenka, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

PDZ

Promoter

hUbC

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only
pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Expression

Mutation

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

Plasmid

#146503

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

Plasmid

#91918

Purpose

Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbfCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pC032 - RcdCas13a from Rhodobacter capsulatus DE442

Plasmid

#91922

Purpose

Expresses RcdCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcdCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC030 - RcsCas13a from Rhodobacter capsulatus SB 1003

Plasmid

#91920

Purpose

Expresses RcsCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

RcsCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pC023 - CaCas13a from [Clostridium] aminophilum DSM 10710

Plasmid

#91913

Purpose

Expresses CaCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

CaCas13a and guide expression cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta11 Aphrodite

Plasmid

#13569

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta11 Aphrodite

Use

Tags

Aphrodite and ECFP

Expression

Bacterial

Mutation

Fluorophore-derived linker shortened. F16A mutati…

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pNB784

Plasmid

#60163

Purpose

Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc-yEVenus

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

SIC1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PRKRA

Plasmid

#106109

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting PRKRA (PRKRA Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDEST_TO_EWSR1

Plasmid

#26376

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-CT

Plasmid

#18069

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Use

Tags

Expression

Insect

Mutation

P418S

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Use

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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FHp5pUP95C3,5SGW (E5)

Plasmid

#74035

Purpose

lentiviral expression of Psd95 shRNA and Psd95-C3,5S-EGFP fusion

Depositor

Oliver Schluter, Robert Malenka, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

PCR: C3,5S (tct aga cca cca tgg act Ctc tAt CGa t…

Promoter

H1

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK IRF9 3'UTR

Plasmid

#19863

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

IRF9 3'UTR (IRF9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

CMVpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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