We narrowed to 1,020 results for: TSK

1. Lenti-LucAL

   Plasmid

   #174049

   Purpose

   Expresses mALG8 (ITYTWTRL) and mLAMA4 (VGFNFRTL) antigens as a fusion to luciferase and Cre recombinase

   Depositor

   Tyler Jacks

   Article

   Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

   Insert

   LucAL
   

   Use

   Cre/Lox, Lentiviral, and Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   ALG8 alanine 506 to threonine; LAMA4 glycine 1254…

   Promoter

   Human Ubiquitin C

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

   Plasmid

   #52299

   Purpose

   Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   15-bp deletion in the promoter

   Promoter

   Human GPR56 e1m promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQCXIZ GFP PODXL delta PBM

   Plasmid

   #202415

   Purpose

   Expression of GFP-tagged PODXL PDZ-binding motif mutation (PBM; doesn't bind NHERF1/2)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Lacking DTHL

   Promoter

   Available since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQCXIZ GFP PODXL FBM

   Plasmid

   #202417

   Purpose

   Expression of GFP-tagged PODXL FERM-binding motif mtuation (FBM; doesn't bind Ezrin)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   H477/R479/S481>AAA mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV2-UbC-3xNLS-mScarlet-I

   Plasmid

   #191099

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet-I
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   optimized to human codon usage

   Promoter

   UbC

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mTet1 flox donor

   Plasmid

   #117147

   Purpose

   Making Tet1 conditional knock out

   Depositor

   Jacob Hanna

   Article

   Zviran et al ssrn.3155731

   Insert

   Tet1 (Tet1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYX233-b2delta-DHFR

   Plasmid

   #163760

   Purpose

   Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   b2delta-DHFR (Dhfr Budding Yeast, Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

   Promoter

   GAL1

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX303 TagRFP-T LGALS3 R186S

   Plasmid

   #202428

   Purpose

   Expression of TagRFP-T-tagged Galectin 3 with mutation of carbohdrate recognition binding domain (CRD) (R186S mutant)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Lentiviral

   Tags

   TagRFP

   Expression

   Mutation

   R186S

   Promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUAP1

   Plasmid

   #63674

   Purpose

   Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

   Depositor

   Nicola Patron

   Article

   Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

   Insert

   RFP cloning selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB-GG-OMKSL-PGK-Puro

    Plasmid

    #102894

    Purpose

    PiggyBac transposon system construct with 10 concatenated U6 promoter driven transcriptional cassettes targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Contains PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OMKSL-gRNAs-PGK-Puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid

    #102909

    Purpose

    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GG-EBNA-OMKSL-PP

    Plasmid

    #102899

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    OMKSL-gRNAS-PGK-puro (POU5F1, MYC, KLF4, SOX2, LIN28A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcGFP-IVT

    Plasmid

    #205145

    Purpose

    IVT template of AcGFP with MCS in frame and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcGFP-stop-IVT

    Plasmid

    #205146

    Purpose

    IVT template of AcGFP stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    AcGFP1
    

    Use

    In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRLuc-IVT

    Plasmid

    #205148

    Purpose

    IVT template of Renilla luciferase stop codon between the ORF and MCS and polyA

    Depositor

    Igor Ulitsky

    Insert

    Renilla Luciferase
    

    Use

    Luciferase; In-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLentiV2-dCas9-VP64

    Plasmid

    #141104

    Purpose

    Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

    Depositor

    Igor Ulitsky

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSK-mPar1

    Plasmid

    #53227

    Purpose

    bacterial expression of mouse Par1

    Depositor

    Shaun Coughlin

    Insert

    Par1 (F2r Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pRS300

    Plasmid

    #22846

    Purpose

    Backbone for expressing plant artificial miRNAs

    Depositor

    Detlef Weigel

    Article

    Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

    Insert

    MIR319a (MIR319a Mustard Weed)
    

    Use

    RNAi; Plant gene inactivation

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p663-UBC-miniTurbo-V5-TSSK2_IDG-K

    Plasmid

    #189894

    Purpose

    Gateway destination clone of TSSK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    TSSK2 (TSSK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p663-UBC-miniTurbo-V5-TSSK3_IDG-K

    Plasmid

    #170617

    Purpose

    Gateway destination clone of TSSK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TSSK3 (TSSK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge