We narrowed to 1,958 results for: cp

1. pCAG CAPN7 V18D-OSF

   Plasmid

   #193042

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with V18D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   V18D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG CAPN7 L61D-OSF

   Plasmid

   #193043

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with L61D mutation. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   L61D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG CAPN7 F98D-OSF

   Plasmid

   #193044

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with F98D mutation that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   F98D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG CAPN7 V18D,F98D-OSF

   Plasmid

   #193045

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   V18D, F98D

   Promoter

   Chicken Beta-actin

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-CAPN7-V18D-mCherry

   Plasmid

   #193046

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with V18D (IST1-binding mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   V18D, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-CAPN7-C290S-mCherry

   Plasmid

   #193047

   Purpose

   Lentiviral expression vector for stable integration of inducible, siRNA-resistant CAPN7 with C290S (proteolysis inactivating mutation) with C-terminal mCherry tag.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C290S, siRNA resistant to: GCACCCAUACCUUUACAUU

   Promoter

   Tight TRE

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CAPN7 L61D (1-165)

   Plasmid

   #193033

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L61D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CAPN7 V18D,F98D (1-165)

   Plasmid

   #193034

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with V18D and F98D mutations that abolish binding to IST1.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   V18D, F98D, Residues 1-165

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTMB398_ZFcharm_Prnp_SMM

   Plasmid

   #220845

   Purpose

   AAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpyTag003(V114T, V116T)-Talin rod-mCherry

    Plasmid

    #210027

    Purpose

    Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    SpyTag003(V114T,V116T)-Talin1 rod domain(434-2541)-mCherry
    

    Expression

    Mammalian

    Mutation

    SpyTag003 V114T and V116T mutations to modify Spy…

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LifeAct-mNeonGreen -IRES- Talin head-SpyTag003

    Plasmid

    #210024

    Purpose

    Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    LifeAct-mNeonGreen co-expressed with Talin head(1-433)-SpyTag003
    

    Expression

    Mammalian

    Mutation

    Encephalomyocarditis virus internal ribosome entr…

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Talin head-SpyCatcher003(K31TAG)

    Plasmid

    #210023

    Purpose

    Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

    Depositor

    Vesa Hytönen

    Article

    Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

    Insert

    EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
    

    Expression

    Mammalian

    Mutation

    SpyCatcher003 with K31TAG (Amber stop codon) muta…

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJGL003

    Plasmid

    #180607

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX+Region3 insertion

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-f1(-)-TOMM20-dTomato

    Plasmid

    #115922

    Purpose

    donor to insert dTomato at the TOMM20 locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    TOMM20-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-f1(+)-GAPDH-IRES-dTomato

    Plasmid

    #115912

    Purpose

    donor to insert IRES-dTomato at the GAPDH locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    IRES-dTomato
    

    Use

    Donor template

    Tags

    dTomato

    Available Since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMY-CD90.2-P2A-BlastDYK

    Plasmid

    #163349

    Purpose

    Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMX-mPGK-CD3-P2A-CD90.2

    Plasmid

    #163337

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the mPGK promoter

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    mPGK

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMX-hFTH1-CD3-P2A-CD90.2

    Plasmid

    #163335

    Purpose

    Retroviral vector to overexpress the murine CD3 (TCR) components CD3 gamma, CD delta, CD epsilon and TCR zeta and surface marker CD90.2, separated by T2; F2A; E2A; P2A respectively, under control of the hFTH1 promoter

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD3
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    hFTH1

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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