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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Trim46

Plasmid

#176402

Purpose

TRIM46 tagged with GFP

Depositor

Thomas Schwarz, Casper Hoogenraad

Article

van Beuningen et al Neuron. 2015 Dec 16;88(6):1208-1226. doi

Insert

trim46 (Trim46 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.dEZH2

Plasmid

#220241

Purpose

Expression of an EZH2 catalytic site mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

dEZH2 (EZH2 Human)

Use

Tags

6x His, MBP

Expression

Insect

Mutation

Y731F

Promoter

Polyhedrin

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1 ElonginB, ElonginC

Plasmid

#230999

Purpose

Dual expression vector containing mouse ElonginB and ElonginC for E.coli protein purification

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Elongin B (Elob Mouse)
Elongin C (Eloc Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRecLV105 MEF2C

Plasmid

#107240

Purpose

A lentiviral vector for expression in mammalian cells encoding MEF2C insert

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pRecLV105 MEF2C S222A

Plasmid

#107239

Purpose

A lentiviral vector for expression in mammalian cells encoding MEF2C-S222A insert

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C S222A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MLL2 653

Plasmid

#11017

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MLL2 (KMT2B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

truncation of MLL2, last 653 amino acids, from pr…

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-HA-mBec1 F121A

Plasmid

#99506

Purpose

Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

Depositor

Congcong He

Article

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Expression

Mutation

F121A

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHIV-EGFP.Flag-EZH2-mt2*

Plasmid

#220246

Purpose

Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2* (EZH2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

PRKKKR494-499NAAIRS

Promoter

EF-1α

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EPOP

Plasmid

#125170

Purpose

Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EPOP (EPOP Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Puro dn-cHSF1

Plasmid

#134728

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222D

Plasmid

#107234

Purpose

MEF2C phosphomimetic mutant serine to aspartic acid

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(TBK1)

Plasmid

#109322

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (TBK1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.CBX8ΔChromo

Plasmid

#224708

Purpose

Expresses a human CBX8 truncation (deletion of the chromodomain) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 chromodomain truncation (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL.mEGFP-CBX8

Plasmid

#224710

Purpose

Expresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Use

Tags

mEGFP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Oct. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Use

Tags

HMBP

Expression

Insect

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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