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pFUW H2B-mEmerald-P2A-PuroR

Plasmid

#239553

Purpose

Expression and nuclear localization of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMCB306

Plasmid

#89360

Purpose

sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

GFP, puromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

Plasmid

#233540

Purpose

A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L1 (TCF7L1 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-NeoR

Plasmid

#239570

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBAC-ECFP-15xQUAS_TATA-SV40

Plasmid

#104875

Purpose

piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

Depositor

Christopher Potter

Article

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGL198

Plasmid

#180472

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

ZmCas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-2

Plasmid

#42211

Purpose

A genetically encoded sensor for H2O2 with expanded dynamic range.

Depositor

Vsevolod Belousov

Article

Markvicheva et al Bioorg Med Chem. 2011 Feb 1;19(3):1079-8

Insert

HYPER 2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pγsat

Plasmid

#39238

Depositor

Niall Dillon

Article

Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Insert

8 copies of the 234bp mouse γ-satellite repeat

Use

Cloning vector

Available Since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMF1754

Plasmid

#125585

Purpose

Expresses peroxisomal KillerRed (po-KR) in mammalian cells

Depositor

Marc Fransen

Article

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

po-KillerRed

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4-TO-Hygromycin-mVenus-MAP

Plasmid

#44099

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Tags

mVenus-MAP

Expression

Mammalian

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-TIS11B CPM2

Plasmid

#121163

Purpose

Mammalian expression of GFP tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-PuroR

Plasmid

#239566

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 NM23-H1

Plasmid

#10956

Depositor

Patricia Steeg

Article

Steeg et al J Natl Cancer Inst. 1988 Apr 6. 80(3):20

Insert

NM23 (NME1 Human)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pGL193

Plasmid

#180471

Purpose

For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

Depositor

Peggy Lemaux

Article

Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Cas9

Expression

Plant

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBA2kIKmA2

Plasmid

#39213

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Kudzu Isoprene Synthase

Use

Synthetic Biology

Mutation

Deleted the predicted chloroplast transit peptide

Promoter

psbA2

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
hOCT1-miRFP

Plasmid

#188995

Purpose

A mammalian expression plasmid encoding miRFP-tagged human OCT1

Depositor

Jeremy Baskin

Article

Chiu et al JACS Au. 2022 Nov 28;2(12):2703-2713. do

Insert

hOCT1 (SLC22A1 Human)

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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