We narrowed to 1,598 results for: pfas

1. pv7_CBX1_2xChromo_noEGFP

   Plasmid

   #179395

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFP

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo_noEGFP
   

   Use

   Tags

   AviTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pv6_MBD1_MBD-R22A

   Plasmid

   #179405

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBD(R22A)

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1_MBD(R22A)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   MBD R22A

   Promoter

   CAGGS

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-HTNC

   Plasmid

   #13763

   Purpose

   For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.

   Depositor

   Klaus Rajewsky

   Article

   Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

   Insert

   His-TAT-NLS-Cre
   

   Use

   Baculovirus

   Tags

   His Tag, NLS, and TAT

   Expression

   Bacterial

   Mutation

   Promoter

   p10 promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. IMPT-10314:GABBR2

   Plasmid

   #157851

   Purpose

   HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

   Depositor

   Raymond Stevens, Vadim Cherezov

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABBR2 (GABBR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1-PHD2

   Plasmid

   #21401

   Depositor

   Eric Metzen

   Article

   Metzen et al J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-2

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

6. IMPT-2070

   Plasmid

   #172092

   Purpose

   pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

   Depositor

   Raymond Stevens

   Article

   Liu et al Science. 2012 Jul 13;337(6091):232-6. do

   Insert

   Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)
   

   Use

   Tags

   cytochrome b562RIL

   Expression

   Insect

   Mutation

   Promoter

   gp64

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pv9_CBX7_2xChromo-13XL-BASU

   Plasmid

   #179413

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.2xChromo-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX7.2xChromo-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pv9_MBD1_MBD-WT-13XL-BASU

   Plasmid

   #179416

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1.MBD-WT-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pv9_TAF3_2xPHD-13XL-BASU

   Plasmid

   #179414

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   TAF3.2xPHD-13XL-BASU
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pv9_CBX1_2xChromo-13XL-BASU

    Plasmid

    #179412

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1.2xChromo-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1.2xChromo-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Sandgrouse+R111Q–insD

    Plasmid

    #191229

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    removed aspartic acid at 121 and replaced arginin…

    Promoter

    PH and p10

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pv6_CBX7_Chromo-W35A_TAF3_Phd

    Plasmid

    #179401

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo(W35A) - TAF3_Phd

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo(W35A) - TAF3_Phd
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Chromodomain W35A

    Promoter

    CAGGS

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pv9_CBX7_Chromo/TAF3.PHD-13XL-BASU

    Plasmid

    #179415

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7.Chromo/TAF3.PHD-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7.Chromo/TAF3.PHD-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. kanMX6-ins6

    Plasmid

    #195043

    Purpose

    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RAT

    Plasmid

    #187416

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    PH and p10

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. natMX-ins7

    Plasmid

    #195044

    Purpose

    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    NatR
    

    Use

    Yeast genomic targeting

    Tags

    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pv9_MBD1_MBD-R22A-13XL-BASU

    Plasmid

    #179417

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-R22A-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    MBD1.MBD-R22A-13XL-BASU
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pv6_CBX7_Chromo_TAF3_Phd-DW890/891AA

    Plasmid

    #179402

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_Chromo - TAF3_Phd(DW890/891AA)

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX7_Chromo - TAF3_Phd(DW890/891AA)
    

    Use

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Mutation

    Phd domain DW890/891AA

    Promoter

    CAGGS

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. scAAV-sgRNA-GFP

    Plasmid

    #177935

    Purpose

    Expresses sgRNA and can be packaged into AAV particles for somatic delivery into Cas9 transgenic mice

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge