We narrowed to 5,093 results for: puc

1. pUC-DEST-cjSTELLA-mCerulean-PuroTK

   Plasmid

   #141029

   Purpose

   Reporter integration

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   mCerulean
   

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Prham-tagRFP-IMBB2.4-pUC57-mini

   Plasmid

   #149343

   Purpose

   expresses tagRFP fluorescent protein in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

   Insert

   tagRFP
   

   Expression

   Bacterial

   Promoter

   rhamnose

   Available Since

   June 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

   Plasmid

   #133791

   Purpose

   U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St1Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   V1 sgRNA architecture from Carter et al. biorxiv …

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

   Plasmid

   #133792

   Purpose

   U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   St3Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Muller et al. Molecular T…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCHR-CD5HA2-P2A-smAID-mClover

   Plasmid

   #110658

   Purpose

   Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CD5HA2-P2A-AID-mClover
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MoClo adapted pUC57-Kan Level 2

   Plasmid

   #109218

   Purpose

   Non binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants

   Depositor

   Elena Caro Bernat

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57 DYSF, alt exon 1, 40a

   Plasmid

   #87894

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 40a (DYSF Human)
   

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57 DYSF, alt exon 1, 5a, 17, 40a

   Plasmid

   #87895

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1, 5a, 17, 40a (DYSF Human)
   

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57 DYSF, alt exon 1a, 40a

   Plasmid

   #87901

   Purpose

   research of alternative isoforms of the protein dysferlin

   Depositor

   Jain Foundation

   Insert

   DYSF, alt exon 1a, 40a (DYSF Human)
   

   Expression

   Bacterial

   Mutation

   alternative isoforms, see comments

   Available Since

   March 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC57 DYSF, alt exon 1a, 17, 40a

    Plasmid

    #87899

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1a, 17, 40a (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57 DYSF, alt exon 1, 17, 40a

    Plasmid

    #87891

    Purpose

    research of alternative isoforms of the protein dysferlin

    Depositor

    Jain Foundation

    Insert

    DYSF, alt exon 1, 17, 40a (DYSF Human)
    

    Expression

    Bacterial

    Mutation

    alternative isoforms, see comments

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19 gene 60 thru gap + Cys

    Plasmid

    #49161

    Purpose

    Template for generating gene 60 mRNA truncated two codons after coding gap + Cys

    Depositor

    Nils Walter

    Article

    Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

    Insert

    Bacteriophage T4 gene 60
    

    Mutation

    gene 60 mRNA truncated two codons after coding ga…

    Promoter

    T7

    Available Since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB-GA-mNeonGreen-linker-attB-GT::pUC-GW-Kan

    Plasmid

    #244898

    Purpose

    Recombinase-mediated integration

    Depositor

    Andrew Bassett

    Article

    Migliori et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

    Insert

    mNeonGreen
    

    Use

    Unspecified

    Available Since

    Nov. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid

    #215769

    Purpose

    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus

    Depositor

    Dimitrios Wagner

    Article

    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:

    Inserts

    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Human, Mouse)
    TRAC-3' homology arm (TRAC Human)
    

    Expression

    Bacterial

    Mutation

    Cas12a PAM mutated

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

    Plasmid

    #154824

    Purpose

    (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    Peft-3::wrmScarlet::tbb-2 UTR
    

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-TgKI-MCS-eGFP_Y40N-MCS-polyA

    Plasmid

    #174025

    Purpose

    This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

    Depositor

    Michel Bagnat

    Article

    Levic et al Development. 2021 Sep 8. pii: 272090. do

    Insert

    eGFP
    

    Use

    Zebrafish knock-in tagging

    Mutation

    Y40N

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTR-345 pUC19-tNGFR-P2A-IL2RB N

    Plasmid

    #186064

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RB (IL2RB Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTR-340 pUC19-tNGFR-P2A-IL2RG N

    Plasmid

    #186067

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL2RG (IL2RG Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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